6LMH

Structure of an ASFV-derived histone-like protein pA104R

6LMH の概要

• エントリーDOI: 10.2210/pdb6lmh/pdb
• 分子名称: pA104R (1 entity in total)
• 機能のキーワード: apo state, dna binding protein
• 由来する生物種: African swine fever virus (ASFV)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 24919.27

構造登録者

Wang, H.,Qi, J.,Chai, Y.,Gao, F. (登録日: 2019-12-25, 公開日: 2020-05-13, 最終更新日: 2024-03-27)

主引用文献

Liu, R.,Sun, Y.,Chai, Y.,Li, S.,Li, S.,Wang, L.,Su, J.,Yu, S.,Yan, J.,Gao, F.,Zhang, G.,Qiu, H.J.,Gao, G.F.,Qi, J.,Wang, H.
The structural basis of African swine fever virus pA104R binding to DNA and its inhibition by stilbene derivatives.
Proc.Natl.Acad.Sci.USA, 117:11000-11009, 2020

PubMed Abstract: African swine fever virus (ASFV) is a highly contagious nucleocytoplasmic large DNA virus (NCLDV) that causes nearly 100% mortality in swine. The development of effective vaccines and drugs against this virus is urgently needed. pA104R, an ASFV-derived histone-like protein, shares sequence and functional similarity with bacterial HU/IHF family members and is essential for viral replication. Herein, we solved the crystal structures of pA104R in its apo state as well as in complex with DNA. Apo-pA104R forms a homodimer and folds into an architecture conserved in bacterial heat-unstable nucleoid proteins/integration host factors (HUs/IHFs). The pA104R-DNA complex structure, however, uncovers that pA104R has a DNA binding pattern distinct from its bacterial homologs, that is, the β-ribbon arms of pA104R stabilize DNA binding by contacting the major groove instead of the minor groove. Mutations of the basic residues at the base region of the β-strand DNA binding region (BDR), rather than those in the β-ribbon arms, completely abolished DNA binding, highlighting the major role of the BDR base in DNA binding. An overall DNA bending angle of 93.8° is observed in crystal packing of the pA104R-DNA complex structure, which is close to the DNA bending angle in the HU-DNA complex. Stilbene derivatives SD1 and SD4 were shown to disrupt the binding between pA104R and DNA and inhibit the replication of ASFV in primary porcine alveolar macrophages. Collectively, these results reveal the structural basis of pA104R binding to DNA highlighting the importance of the pA104R-DNA interaction in the ASFV replication cycle and provide inhibitor leads for ASFV chemotherapy.

PubMed: 32358196
DOI: 10.1073/pnas.1922523117

実験手法

X-RAY DIFFRACTION (2.805 Å)