6LLN

citronellol catabolism dehydrogenase (AtuB) [Pseudomonas aeruginosa PAO1]

6LLN の概要

• エントリーDOI: 10.2210/pdb6lln/pdb
• 分子名称: Putative dehydrogenase involved in catabolism of citronellol (2 entities in total)
• 機能のキーワード: acyclic terpene utilization pathway, atub, short-chain dehydrogenase/reductase(sdr) family., oxidoreductase
• 由来する生物種: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 30786.85

構造登録者

Zhang, Q.,Bartlam, M. (登録日: 2019-12-23, 公開日: 2020-02-05, 最終更新日: 2023-11-22)

主引用文献

Chen, Y.,Jia, H.,Liang, Y.,Zhang, H.,Che, S.,Liu, R.,Zhang, Q.,Bartlam, M.
Structural characterization of the Pseudomonas aeruginosa dehydrogenase AtuB involved in citronellol and geraniol catabolism.
Biochem.Biophys.Res.Commun., 523:954-960, 2020

PubMed Abstract: Pseudomonas aeruginosa can metabolize acyclic monoterpenoids (such as citronellol and geraniol) as the only carbon and energy sources. A total of seven proteins (AtuA, AtuB, AtuCF, AtuD, AtuE, AtuG, AtuH) have been identified in Pseudomonas aeruginosa as participating in the acyclic terpene utilization pathway. AtuB is a dehydrogenase enzyme responsible for citronellol and geraniol catabolism in the acyclic terpene utilization (Atu) pathway, although its structure and function have not been characterized to date. Here we report the crystal structure of AtuB from Pseudomonas aeruginosa PAO1 (PaAtuB) to 1.8 Å resolution. PaAtuB crystallizes in the space group F222 with a single monomer in the asymmetric unit. Analytical ultracentrifugation data shows that PaAtuB forms a stable tetramer in solution, which is consistent with the structure. Structural analysis confirms that AtuB belongs to the short-chain dehydrogenase/reductase (SDR) family. AtuB is predicted to bind NADP(H) from the crystal structure, which is confirmed by MicroScale Thermophoresis analysis that shows PaAtuB binds NADP(H) with a Kd value of 258 μM. This work provides a starting point to explore potential biotechnology and pharmaceutical applications of AtuB.

PubMed: 31964529
DOI: 10.1016/j.bbrc.2020.01.052

実験手法

X-RAY DIFFRACTION (1.8 Å)