6LJF

Crystal structure of gelsolin G3 domain (calcium condition)

6LJF の概要

• エントリーDOI: 10.2210/pdb6ljf/pdb
• 分子名称: Gelsolin, CALCIUM ION, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: fragmin, gelsolin family protein, calcium regulation, actin filament severing, cytosolic protein
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 22784.05

構造登録者

Takeda, S. (登録日: 2019-12-14, 公開日: 2020-01-01, 最終更新日: 2023-11-22)

主引用文献

Takeda, S.,Fujiwara, I.,Sugimoto, Y.,Oda, T.,Narita, A.,Maeda, Y.
Novel inter-domain Ca2+-binding site in the gelsolin superfamily protein fragmin.
J.Muscle Res.Cell.Motil., 41:153-162, 2020

PubMed Abstract: Gelsolin superfamily proteins, consisting of multiple domains (usually six), sever actin filaments and cap the barbed ends in a Ca-dependent manner. Two types of evolutionally conserved Ca-binding sites have been identified in this family; type-1 (between gelsolin and actin) and type-2 (within the gelsolin domain). Fragmin, a member in the slime mold Physarum polycephalum, consists of three domains (F1-F3) that are highly similar to the N-terminal half of mammalian gelsolin (G1-G3). Despite their similarities, the two proteins exhibit a significant difference in the Ca dependency; F1-F3 absolutely requires Ca for the filament severing whereas G1-G3 does not. In this study, we examined the strong dependency of fragmin on Ca using biochemical and structural approaches. Our co-sedimentation assay demonstrated that Ca significantly enhanced the binding of F2-F3 to actin. We determined the crystal structure of F2-F3 in the presence of Ca. F2-F3 binds a total of three calcium ions; while two are located in type-2 sites within F2 or F3, the remaining one resides between the F2 long helix and the F3 short helix. The inter-domain Ca-coordination appears to stabilize F2-F3 in a closely packed configuration. Notably, the F3 long helix exhibits a bent conformation which is different from the straight G3 long helix in the presence of Ca. Our results provide the first structural evidence for the existence of an unconventional Ca-binding site in the gelsolin superfamily proteins.

PubMed: 31863323
DOI: 10.1007/s10974-019-09571-5

実験手法

X-RAY DIFFRACTION (1.5 Å)