6LIX

CRL Protein of Arabidopsis

6LIX の概要

• エントリーDOI: 10.2210/pdb6lix/pdb
• 分子名称: Chromophore lyase CRL, chloroplastic, 2-(N-MORPHOLINO)-ETHANESULFONIC ACID (3 entities in total)
• 機能のキーワード: a homolog of cyanobacterial cpct lyase, plant protein, lyase
• 由来する生物種: Arabidopsis thaliana (Mouse-ear cress)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 61128.76

構造登録者

Wang, F.F.,Guan, K.L.,Sun, P.K.,Xing, W.M. (登録日: 2019-12-13, 公開日: 2020-09-16, 最終更新日: 2024-10-30)

主引用文献

Wang, F.,Fang, J.,Guan, K.,Luo, S.,Dogra, V.,Li, B.,Ma, D.,Zhao, X.,Lee, K.P.,Sun, P.,Xin, J.,Liu, T.,Xing, W.,Kim, C.
The Arabidopsis CRUMPLED LEAF protein, a homolog of the cyanobacterial bilin lyase, retains the bilin-binding pocket for a yet unknown function.
Plant J., 104:964-978, 2020

PubMed Abstract: The photosynthetic bacterial phycobiliprotein lyases, also called CpcT lyases, catalyze the biogenesis of phycobilisome, a light-harvesting antenna complex, through the covalent attachment of chromophores to the antenna proteins. The Arabidopsis CRUMPLED LEAF (CRL) protein is a homolog of the cyanobacterial CpcT lyase. Loss of CRL leads to multiple lesions, including localized foliar cell death, constitutive expression of stress-related nuclear genes, abnormal cell cycle, and impaired plastid division. Notwithstanding the apparent phenotypes, the function of CRL still remains elusive. To gain insight into the function of CRL, we examined whether CRL still retains the capacity to bind with the bacterial chromophore phycocyanobilin (PCB) and its plant analog phytochromobilin (PΦB). The revealed structure of the CpcT domain of CRL is comparable to that of the CpcT lyase, despite the low sequence identity. The subsequent in vitro biochemical assays found, as shown for the CpcT lyase, that PCB/PΦB binds to the CRL dimer. However, some mutant forms of CRL, substantially compromised in their bilin-binding ability, still restore the crl-induced multiple lesions. These results suggest that although CRL retains the bilin-binding pocket, it seems not functionally associated with the crl-induced multiple lesions.

PubMed: 32860438
DOI: 10.1111/tpj.14974

実験手法

X-RAY DIFFRACTION (2.385 Å)