6LHT

Localized reconstruction of coxsackievirus A16 mature virion in complex with Fab 18A7

Summary for 6LHT

• Entry DOI: 10.2210/pdb6lht/pdb
• EMDB information: 0897
• Descriptor: light chain variable region of Fab 18A7, heavy chain variable region of Fab 18A7, VP1 protein, ... (4 entities in total)
• Functional Keywords: localized reconstruction, viral protein
• Biological source: Mus musculus; More
• Total number of polymer chains: 7
• Total formula weight: 193038.04

Authors

He, M.Z.,Xu, L.F.,Zheng, Q.B.,Zhu, R.,Yin, Z.C.,Cheng, T.,Li, S.W. (deposition date: 2019-12-10, release date: 2020-02-05, Last modification date: 2024-10-23)

Primary citation

He, M.,Xu, L.,Zheng, Q.,Zhu, R.,Yin, Z.,Zha, Z.,Lin, Y.,Yang, L.,Huang, Y.,Ye, X.,Li, S.,Hou, W.,Wu, Y.,Han, J.,Liu, D.,Li, Z.,Chen, Z.,Yu, H.,Que, Y.,Wang, Y.,Yan, X.,Zhang, J.,Gu, Y.,Zhou, Z.H.,Cheng, T.,Li, S.,Xia, N.
Identification of Antibodies with Non-overlapping Neutralization Sites that Target Coxsackievirus A16.
Cell Host Microbe, 27:249-, 2020

PubMed Abstract: Hand, foot, and mouth disease is a common childhood illness primarily caused by coxsackievirus A16 (CVA16), for which there are no current vaccines or treatments. We identify three CVA16-specific neutralizing monoclonal antibodies (nAbs) with therapeutic potential: 18A7, 14B10, and NA9D7. We present atomic structures of these nAbs bound to all three viral particle forms-the mature virion, A-particle, and empty particle-and show that each Fab can simultaneously occupy the mature virion. Additionally, 14B10 or NA9D7 provide 100% protection against lethal CVA16 infection in a neonatal mouse model. 18A7 binds to a non-conserved epitope present in all three particles, whereas 14B10 and NA9D7 recognize broad protective epitopes but only bind the mature virion. NA9D7 targets an immunodominant site, which may overlap the receptor-binding site. These findings indicate that CVA16 vaccines should be based on mature virions and that these antibodies could be used to discriminate optimal virion-based immunogens.

PubMed: 32027857
DOI: 10.1016/j.chom.2020.01.003

Experimental method

ELECTRON MICROSCOPY (3.67 Å)