6LGX

Structure of Rabies virus glycoprotein at basic pH

6LGX の概要

• エントリーDOI: 10.2210/pdb6lgx/pdb
• 分子名称: Glycoprotein,Glycoprotein,Glycoprotein (1 entity in total)
• 機能のキーワード: functional class, viral protein
• 由来する生物種: Rabies lyssavirus; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 99266.56

構造登録者

Yang, F.L.,Lin, S.,Ye, F.,Yang, J.,Qi, J.X.,Chen, Z.J.,Lin, X.,Wang, J.C.,Yue, D.,Cheng, Y.W.,Chen, Z.M.,Chen, H.,You, Y.,Zhang, Z.L.,Yang, Y.,Yang, M.,Sun, H.L.,Li, Y.H.,Cao, Y.,Yang, S.Y.,Wei, Y.Q.,Gao, G.F.,Lu, G.W. (登録日: 2019-12-06, 公開日: 2020-02-19, 最終更新日: 2024-10-09)

主引用文献

Yang, F.,Lin, S.,Ye, F.,Yang, J.,Qi, J.,Chen, Z.,Lin, X.,Wang, J.,Yue, D.,Cheng, Y.,Chen, Z.,Chen, H.,You, Y.,Zhang, Z.,Yang, Y.,Yang, M.,Sun, H.,Li, Y.,Cao, Y.,Yang, S.,Wei, Y.,Gao, G.F.,Lu, G.
Structural Analysis of Rabies Virus Glycoprotein Reveals pH-Dependent Conformational Changes and Interactions with a Neutralizing Antibody.
Cell Host Microbe, 27:441-, 2020

PubMed Abstract: Rabies virus (RABV), the etiological agent for the lethal disease of rabies, is a deadly zoonotic pathogen. The RABV glycoprotein (RABV-G) is a key factor mediating virus entry and the major target of neutralizing antibodies. Here, we report the crystal structures of RABV-G solved in the free form at ∼pH-8.0 and in the complex form with a neutralizing antibody 523-11 at ∼pH-6.5, respectively. RABV-G has three domains, and the basic-to-acidic pH change results in large domain re-orientations and concomitant domain-linker re-constructions, switching it from a bent hairpin conformation into an extended conformation. During such low-pH-induced structural transitions, residues located in the domain-linker are found to play important roles in glycoprotein-mediated membrane fusion. Finally, the antibody interacts with RABV-G mainly through its heavy chain and binds to a bipartite conformational epitope in the viral protein for neutralization. These structures provide valuable information for vaccine and drug design.

PubMed: 32004500
DOI: 10.1016/j.chom.2019.12.012

実験手法

X-RAY DIFFRACTION (3.097 Å)