6LFP

Cry3Aa protein for enzyme entrapment

Summary for 6LFP

Entry DOI	10.2210/pdb6lfp/pdb
Descriptor	Cry3Aa protein (1 entity in total)
Functional Keywords	pesticidal, crystal forming, nanoporous, protein entrapment, toxin
Biological source	Bacillus thuringiensis
Total number of polymer chains	1
Total formula weight	73177.16
Authors	Heater, B.S.,Chan, M.K. (deposition date: 2019-12-03, release date: 2020-10-14, Last modification date: 2023-11-22)
Primary citation	Heater, B.S.,Yang, Z.,Lee, M.M.,Chan, M.K. In Vivo Enzyme Entrapment in a Protein Crystal. J.Am.Chem.Soc., 142:9879-9883, 2020 Cited by PubMed Abstract: Cry3Aa is a protein that forms crystals naturally in the bacterium . Here we report that coexpression of Cry3Aa and a lipase without recombinant fusion results in the efficient passive entrapment of the lipase within the nanoporous channels of the resulting crystals. This Cry3Aa crystal-mediated entrapment provides multiple benefits to the lipase including a high enzyme loading, significantly improved thermostability, increased proteolytic resistance, and the ability to be utilized as a recyclable biodiesel catalyst. These characteristics, along with its greatly simplified method of isolation, highlight the potential of Cry3Aa crystal-mediated enzyme entrapment for use in biocatalysis and other biotechnological applications. PubMed: 32407637 DOI: 10.1021/jacs.9b13462 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (3.31 Å)

Structure validation