6LFP

Cry3Aa protein for enzyme entrapment

6LFP の概要

• エントリーDOI: 10.2210/pdb6lfp/pdb
• 分子名称: Cry3Aa protein (1 entity in total)
• 機能のキーワード: pesticidal, crystal forming, nanoporous, protein entrapment, toxin
• 由来する生物種: Bacillus thuringiensis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 73177.16

構造登録者

Heater, B.S.,Chan, M.K. (登録日: 2019-12-03, 公開日: 2020-10-14, 最終更新日: 2023-11-22)

主引用文献

Heater, B.S.,Yang, Z.,Lee, M.M.,Chan, M.K.
In Vivo Enzyme Entrapment in a Protein Crystal.
J.Am.Chem.Soc., 142:9879-9883, 2020

PubMed Abstract: Cry3Aa is a protein that forms crystals naturally in the bacterium . Here we report that coexpression of Cry3Aa and a lipase without recombinant fusion results in the efficient passive entrapment of the lipase within the nanoporous channels of the resulting crystals. This Cry3Aa crystal-mediated entrapment provides multiple benefits to the lipase including a high enzyme loading, significantly improved thermostability, increased proteolytic resistance, and the ability to be utilized as a recyclable biodiesel catalyst. These characteristics, along with its greatly simplified method of isolation, highlight the potential of Cry3Aa crystal-mediated enzyme entrapment for use in biocatalysis and other biotechnological applications.

PubMed: 32407637
DOI: 10.1021/jacs.9b13462

実験手法

X-RAY DIFFRACTION (3.31 Å)