6LF5

The solution structure of ShSPI

6LF5 の概要

• エントリーDOI: 10.2210/pdb6lf5/pdb
• 分子名称: ShSPI (1 entity in total)
• 機能のキーワード: neutrophil elastase inhibitor, toxin
• 由来する生物種: Scolopendra hainanum
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 3960.59

構造登録者

Luan, N.,Rong, M.Q.,Liu, J.X.,Lai, R. (登録日: 2019-11-29, 公開日: 2020-12-02, 最終更新日: 2024-10-23)

主引用文献

Luan, N.,Zhao, Q.,Duan, Z.,Ji, M.,Xing, M.,Zhu, T.,Mwangi, J.,Rong, M.,Liu, J.,Lai, R.
Identification and Characterization of ShSPI, a Kazal-Type Elastase Inhibitor from the Venom of Scolopendra Hainanum .
Toxins, 11:-, 2019

PubMed Abstract: Elastase is a globular glycoprotein and belongs to the chymotrypsin family. It is involved in several inflammatory cascades on the basis of cleaving the important connective tissue protein elastin, and is strictly regulated to a balance by several endogenous inhibitors. When elastase and its inhibitors are out of balance, severe diseases will develop, especially those involved in the cardiopulmonary system. Much attention has been attracted in seeking innovative elastase inhibitors and various advancements have been taken on clinical trials of these inhibitors. Natural functional peptides from venomous animals have been shown to have anti-protease properties. Here, we identified a kazal-type serine protease inhibitor named ShSPI from the cDNA library of the venom glands of . ShSPI showed significant inhibitory effects on porcine pancreatic elastase and human neutrophils elastase with values of 225.83 ± 20 nM and 12.61 ± 2 nM, respectively. Together, our results suggest that ShSPI may be an excellent candidate to develop a drug for cardiopulmonary diseases.

PubMed: 31817486
DOI: 10.3390/toxins11120708

実験手法

SOLUTION NMR