6LE9

The Human Telomeric Nucleosome Displays Distinct Structural and Dynamic Properties

6LE9 の概要

• エントリーDOI: 10.2210/pdb6le9/pdb
• 分子名称: Histone H3.1, Histone H4, Histone H2A type 1-B/E, ... (7 entities in total)
• 機能のキーワード: telomeric dna, nucleosome core particle, dna binding protein, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 176474.08

構造登録者

Soman, A.,Liew, C.W.,Teo, H.L.,Berezhnoy, N.,Korolev, N.,Rhodes, D.,Nordenskiold, L. (登録日: 2019-11-24, 公開日: 2020-04-22, 最終更新日: 2023-11-22)

主引用文献

Soman, A.,Liew, C.W.,Teo, H.L.,Berezhnoy, N.V.,Olieric, V.,Korolev, N.,Rhodes, D.,Nordenskiold, L.
The human telomeric nucleosome displays distinct structural and dynamic properties.
Nucleic Acids Res., 48:5383-5396, 2020

PubMed Abstract: Telomeres protect the ends of our chromosomes and are key to maintaining genomic integrity during cell division and differentiation. However, our knowledge of telomeric chromatin and nucleosome structure at the molecular level is limited. Here, we aimed to define the structure, dynamics as well as properties in solution of the human telomeric nucleosome. We first determined the 2.2 Å crystal structure of a human telomeric nucleosome core particle (NCP) containing 145 bp DNA, which revealed the same helical path for the DNA as well as symmetric stretching in both halves of the NCP as that of the 145 bp '601' NCP. In solution, the telomeric nucleosome exhibited a less stable and a markedly more dynamic structure compared to NCPs containing DNA positioning sequences. These observations provide molecular insights into how telomeric DNA forms nucleosomes and chromatin and advance our understanding of the unique biological role of telomeres.

PubMed: 32374876
DOI: 10.1093/nar/gkaa289

実験手法

X-RAY DIFFRACTION (2.6 Å)