6LCH

Pseudomonas aeruginosa 5086 (PA5086)

6LCH の概要

• エントリーDOI: 10.2210/pdb6lch/pdb
• 分子名称: Sel1 repeat family protein (2 entities in total)
• 機能のキーワード: t6ss, immunity protein, protein binding
• 由来する生物種: Pseudomonas aeruginosa
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 30642.64

構造登録者

She, Z.,Geng, Z. (登録日: 2019-11-19, 公開日: 2020-05-13, 最終更新日: 2024-11-06)

主引用文献

Wen, H.,Geng, Z.,Gao, Z.,She, Z.,Dong, Y.
Characterization of the Pseudomonas aeruginosa T6SS PldB immunity proteins PA5086, PA5087 and PA5088 explains a novel stockpiling mechanism.
Acta Crystallogr.,Sect.F, 76:222-227, 2020

PubMed Abstract: The bacterial type VI secretion system (T6SS) secretes many toxic effectors to gain advantage in interbacterial competition and for eukaryotic host infection. The cognate immunity proteins of these effectors protect bacteria from their own effectors. PldB is a T6SS trans-kingdom effector in Pseudomonas aeruginosa that can infect both prokaryotic and eukaryotic cells. Three proteins, PA5086, PA5087 and PA5088, are employed to suppress the toxicity of PldB-family proteins. The structures of PA5087 and PA5088 have previously been reported, but the identification of further distinctions between these immunity proteins is needed. Here, the crystal structure of PA5086 is reported at 1.90 Å resolution. A structural comparison of the three PldB immunity proteins showed vast divergences in their electrostatic potential surfaces. This interesting phenomenon provides an explanation of the stockpiling mechanism of T6SS immunity proteins.

PubMed: 32356524
DOI: 10.1107/S2053230X2000566X

実験手法

X-RAY DIFFRACTION (1.899 Å)