6L6V

SPO1 Gp44 N-terminal region (1-55)

6L6V の概要

• エントリーDOI: 10.2210/pdb6l6v/pdb
• 分子名称: E3 protein (1 entity in total)
• 機能のキーワード: dna binding, dna mimic, rna polymerase inhibitor, dna binding protein
• 由来する生物種: Bacillus phage SP01
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5862.59

構造登録者

Liu, B.,Wang, Z. (登録日: 2019-10-29, 公開日: 2021-05-05, 最終更新日: 2024-05-15)

主引用文献

Wang, Z.,Wang, H.,Mulvenna, N.,Sanz-Hernandez, M.,Zhang, P.,Li, Y.,Ma, J.,Wang, Y.,Matthews, S.,Wigneshweraraj, S.,Liu, B.
A Bacteriophage DNA Mimic Protein Employs a Non-specific Strategy to Inhibit the Bacterial RNA Polymerase.
Front Microbiol, 12:692512-692512, 2021

PubMed Abstract: DNA mimicry by proteins is a strategy that employed by some proteins to occupy the binding sites of the DNA-binding proteins and deny further access to these sites by DNA. Such proteins have been found in bacteriophage, eukaryotic virus, prokaryotic, and eukaryotic cells to imitate non-coding functions of DNA. Here, we report another phage protein Gp44 from bacteriophage SPO1 of , employing mimicry as part of unusual strategy to inhibit host RNA polymerase. Consisting of three simple domains, Gp44 contains a DNA binding motif, a flexible DNA mimic domain and a random-coiled domain. Gp44 is able to anchor to host genome and interact bacterial RNA polymerase the β and β' subunit, resulting in bacterial growth inhibition. Our findings represent a non-specific strategy that SPO1 phage uses to target different bacterial transcription machinery regardless of the structural variations of RNA polymerases. This feature may have potential applications like generation of genetic engineered phages with Gp44 gene incorporated used in phage therapy to target a range of bacterial hosts.

PubMed: 34149677
DOI: 10.3389/fmicb.2021.692512

実験手法

SOLUTION NMR