6L6B
X-ray structure of human galectin-10 in complex with L-fucose
Summary for 6L6B
| Entry DOI | 10.2210/pdb6l6b/pdb |
| Descriptor | Galectin-10, beta-L-fucopyranose (3 entities in total) |
| Functional Keywords | beta-sandwich structure, lectin, sugar binding protein |
| Biological source | Homo sapiens (Human) |
| Total number of polymer chains | 1 |
| Total formula weight | 16808.17 |
| Authors | Kamitori, S. (deposition date: 2019-10-28, release date: 2020-03-04, Last modification date: 2023-11-22) |
| Primary citation | Itoh, A.,Nonaka, Y.,Nakakita, S.I.,Yoshida, H.,Nishi, N.,Nakamura, T.,Kamitori, S. Structures of human galectin-10/monosaccharide complexes demonstrate potential of monosaccharides as effectors in forming Charcot-Leyden crystals. Biochem.Biophys.Res.Commun., 2020 Cited by PubMed Abstract: The galectins are a family of β-galactoside-specific animal lectins, and have attracted much attention as novel regulators of the immune system. Galectin-10 is well-expressed in eosinophils, and spontaneously forms Charcot-Leyden crystals (CLCs), during prolonged eosinophilic inflammatory reactions, which are frequently observed in eosinophilic diseases. Although biochemical and structural characterizations of galectin-10 have been done, its biological role and molecular mechanism are still unclear, and few X-ray structures of galectin-10 in complex with monosaccharides/oligosaccharides have been reported. Here, X-ray structures of galectin-10 in complexes with seven monosaccharides are presented with biochemical analyses to detect interactions of galectin-10 with monosaccharides/oligosaccharides. Galectin-10 forms a homo-dimer in the face-to-face orientation, and the monosaccharides bind to the carbohydrate recognition site composed of amino acid residues from two galectin-10 molecules of dimers, suggesting that galectin-10 dimer likely captures the monosaccharides in solution and in vivo. d-Glucose, d-allose, d-arabinose, and D-N-acetylgalactosamine bind to the interfaces between galectin-10 dimers in crystals, and they affect the stability of molecular packing in crystals, leading to easy-dissolving of CLCs, and/or inhibiting the formation of CLCs. These monosaccharides may serve as effectors of G10 to form CLCs in vivo. PubMed: 32081418DOI: 10.1016/j.bbrc.2020.02.037 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.802 Å) |
Structure validation
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