6L56

Fe(II) loaded Tegillarca granosa ferritin

Summary for 6L56

• Entry DOI: 10.2210/pdb6l56/pdb
• Descriptor: Ferritin, FE (III) ION, FE (II) ION, ... (5 entities in total)
• Functional Keywords: ferritin, copper, tegillarca granosa, structural genomics, center for eukaryotic structural genomics, cesg, oxidoreductase
• Biological source: Tegillarca granosa (Malaysian cockle)
• Total number of polymer chains: 24
• Total formula weight: 485338.99

Authors

Jiang, Q.Q.,Su, X.R.,Ming, T.H.,Huan, H.S. (deposition date: 2019-10-22, release date: 2019-11-13, Last modification date: 2023-11-22)

Primary citation

Ming, T.H.,Jiang, Q.Q.,Huo, C.,Huan, H.S.,Wu, Y.,Su, C.,Qiu, X.,Lu, C.,Zhou, J.,Li, Y.,Han, J.,Zhang, Z.,Su, X.R.
Structural Insights Into the Effects of Interactions With Iron and Copper Ions on Ferritin From the Blood Clam Tegillarca granosa.
Front Mol Biosci, 9:800008-800008, 2022

PubMed Abstract: In addition to its role as an iron storage protein, ferritin can function as a major detoxification component in the innate immune defense, and Cu ions can also play crucial antibacterial roles in the blood clam, . However, the mechanism of interaction between iron and copper in recombinant ferritin (TgFer) remains to be investigated. In this study, we investigated the crystal structure of TgFer and examined the effects of Fe and Cu ions on the TgFer structure and catalytic activity. The crystal structure revealed that TgFer presented a typically 4-3-2 symmetry in a cage-like, spherical shell composed of 24 identical subunits, featuring highly conserved organization in both the ferroxidase center and the 3-fold channel. Structural and biochemical analyses indicated that the 4-fold channel of TgFer could be serviced as potential binding sites of metal ions. Cu ions appear to bind preferentially with the 3-fold channel as well as ferroxidase site over Fe ions, possibly inhibiting the ferroxidase activity of TgFer. Our results present a structural and functional characterization of TgFer, providing mechanistic insight into the interactions between TgFer and both Fe and Cu ions.

PubMed: 35359603
DOI: 10.3389/fmolb.2022.800008

Experimental method

X-RAY DIFFRACTION (1.85300580472 Å)