6L4Z

Crystal structure of Zika NS2B-NS3 protease with compound 6

6L4Z の概要

• エントリーDOI: 10.2210/pdb6l4z/pdb
• 分子名称: Genome polyprotein, 4-(hydroxymethyl)benzoic acid, ... (9 entities in total)
• 機能のキーワード: viral protease, protease inhibitor complex, viral protein
• 由来する生物種: Zika virus (ZIKV); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 83410.01

構造登録者

Quek, J.P. (登録日: 2019-10-21, 公開日: 2020-07-15, 最終更新日: 2023-11-22)

主引用文献

Quek, J.P.,Liu, S.,Zhang, Z.,Li, Y.,Ng, E.Y.,Loh, Y.R.,Hung, A.W.,Luo, D.,Kang, C.
Identification and structural characterization of small molecule fragments targeting Zika virus NS2B-NS3 protease.
Antiviral Res., 175:104707-104707, 2020

PubMed Abstract: Zika virus (ZIKV) NS2B-NS3 protease is a validated antiviral target as it is essential for maturation of viral proteins. However, its negatively charged active site hinders the development of orthosteric small-molecule inhibitors. Fragment-based drug discovery (FBDD) is a powerful tool to generate novel chemical starting points against difficult drug targets. In this study, we scre ened a fragment compound library against the Zika protease using a primary thermal shift assay and identified twenty-two fragments which (bind to and) stabilize the protease. We then determined the X-ray crystal structures of two hits from different classes, all of which bind to the S1 pocket of the protease. We confirmed that these two fragments bind to the protease without inducing significant conformational changes using solution NMR spectroscopy. These fragment scaffolds serve as the starting point for subsequent lead compound development.

PubMed: 31953156
DOI: 10.1016/j.antiviral.2020.104707

実験手法

X-RAY DIFFRACTION (1.9 Å)