6L2F

Crystal structure of a cupin protein (tm1459, H54AH58A mutant) in copper (Cu) substituted form

6L2F の概要

• エントリーDOI: 10.2210/pdb6l2f/pdb
• 分子名称: Cupin_2 domain-containing protein, COPPER (II) ION, 3,6,9,12,15,18-HEXAOXAICOSANE-1,20-DIOL, ... (5 entities in total)
• 機能のキーワード: artificial metalloenzymes, copper enzyme, cupin, metal binding protein
• 由来する生物種: Thermotoga maritima MSB8
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 27299.17

構造登録者

Fujieda, N.,Ichihashi, H.,Nishikawa, Y.,Kurisu, G.,Itoh, S. (登録日: 2019-10-03, 公開日: 2020-04-01, 最終更新日: 2024-10-30)

主引用文献

Fujieda, N.,Ichihashi, H.,Yuasa, M.,Nishikawa, Y.,Kurisu, G.,Itoh, S.
Cupin Variants as a Macromolecular Ligand Library for Stereoselective Michael Addition of Nitroalkanes.
Angew.Chem.Int.Ed.Engl., 59:7717-7720, 2020

PubMed Abstract: Cupin superfamily proteins (TM1459) work as a macromolecular ligand framework with a double-stranded β-barrel structure ligating to a Cu ion through histidine side chains. Variegating the first coordination sphere of TM1459 revealed that H52A and H54A/H58A mutants effectively catalyzed the diastereo- and enantioselective Michael addition reaction of nitroalkanes to an α,β-unsaturated ketone. Moreover, calculated substrate docking signified C106N and F104W single-point mutations, which inverted the diastereoselectivity of H52A and further improved the stereoselectivity of H54A/H58A, respectively.

PubMed: 32073197
DOI: 10.1002/anie.202000129

実験手法

X-RAY DIFFRACTION (1.23 Å)