6L1S

Crystal structure of DUSP22 mutant_C88S

Summary for 6L1S

• Entry DOI: 10.2210/pdb6l1s/pdb
• Related: 6KMI
• Descriptor: Dual specificity protein phosphatase 22, PHOSPHATE ION (3 entities in total)
• Functional Keywords: dusp22, atypical dusps, cysteine based protein tyrosine phosphatases (cys-based ptps), active site of dusps, hydrolase
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 1
• Total formula weight: 17890.28

Authors

Lai, C.H.,Chang, C.C.,Lyu, P.C. (deposition date: 2019-09-30, release date: 2020-10-28, Last modification date: 2023-11-22)

Primary citation

Lai, C.H.,Chang, C.C.,Chuang, H.C.,Tan, T.H.,Lyu, P.C.
Structural Insights into the Active Site Formation of DUSP22 in N-loop-containing Protein Tyrosine Phosphatases.
Int J Mol Sci, 21:-, 2020

PubMed Abstract: Cysteine-based protein tyrosine phosphatases (Cys-based PTPs) perform dephosphorylation to regulate signaling pathways in cellular responses. The hydrogen bonding network in their active site plays an important conformational role and supports the phosphatase activity. Nearly half of dual-specificity phosphatases (DUSPs) use three conserved residues, including aspartate in the D-loop, serine in the P-loop, and asparagine in the N-loop, to form the hydrogen bonding network, the D-, P-, N-triloop interaction (DPN-triloop interaction). In this study, DUSP22 is used to investigate the importance of the DPN-triloop interaction in active site formation. Alanine mutations and somatic mutations of the conserved residues, D57, S93, and N128 substantially decrease catalytic efficiency (/) by more than 10-fold. Structural studies by NMR and crystallography reveal that each residue can perturb the three loops and induce conformational changes, indicating that the hydrogen bonding network aligns the residues in the correct positions for substrate interaction and catalysis. Studying the DPN-triloop interaction reveals the mechanism maintaining phosphatase activity in N-loop-containing PTPs and provides a foundation for further investigation of active site formation in different members of this protein class.

PubMed: 33053837
DOI: 10.3390/ijms21207515

Experimental method

X-RAY DIFFRACTION (1.3611 Å)