6KZB

Transglutaminase2 complexed with calcium

6KZB の概要

• エントリーDOI: 10.2210/pdb6kzb/pdb
• 分子名称: Protein-glutamine gamma-glutamyltransferase 2, CALCIUM ION, GUANOSINE-5'-DIPHOSPHATE (3 entities in total)
• 機能のキーワード: protein-glutamine gamma-glutamyltransferase 2, transferase
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 233808.11

構造登録者

Park, H.H.,Kim, C.M. (登録日: 2019-09-23, 公開日: 2020-12-09, 最終更新日: 2025-09-17)

主引用文献

Jeong, E.M.,Lee, K.B.,Kim, G.E.,Kim, C.M.,Lee, J.H.,Kim, H.J.,Shin, J.W.,Kwon, M.A.,Park, H.H.,Kim, I.G.
Competitive Binding of Magnesium to Calcium Binding Sites Reciprocally Regulates Transamidase and GTP Hydrolysis Activity of Transglutaminase 2.
Int J Mol Sci, 21:-, 2020

PubMed Abstract: Transglutaminase 2 (TG2) is a Ca-dependent enzyme, which regulates various cellular processes by catalyzing protein crosslinking or polyamination. Intracellular TG2 is activated and inhibited by Ca and GTP binding, respectively. Although aberrant TG2 activation has been implicated in the pathogenesis of diverse diseases, including cancer and degenerative and fibrotic diseases, the structural basis for the regulation of TG2 by Ca and GTP binding is not fully understood. Here, we produced and analyzed a Ca-containing TG2 crystal, and identified two glutamate residues, E437 and E539, as Ca-binding sites. The enzymatic analysis of the mutants revealed that Ca binding to these sites is required for the transamidase activity of TG2. Interestingly, we found that magnesium (Mg) competitively binds to the E437 and E539 residues. The Mg binding to these allosteric sites enhances the GTP binding/hydrolysis activity but inhibits transamidase activity. Furthermore, HEK293 cells transfected with mutant TG2 exhibited higher transamidase activity than cells with wild-type TG2. Cells with wild-type TG2 showed an increase in transamidase activity under Mg-depleted conditions, whereas cells with mutant TG2 were unaffected. These results indicate that E437 and E539 are Ca-binding sites contributing to the reciprocal regulation of transamidase and GTP binding/hydrolysis activities of TG2 through competitive Mg binding.

PubMed: 31991788
DOI: 10.3390/ijms21030791

実験手法

X-RAY DIFFRACTION (3.55 Å)