6KZA

Crystal structure of the complex of the interaction domains of E. coli DnaB helicase and DnaC helicase loader

6KZA の概要

• エントリーDOI: 10.2210/pdb6kza/pdb
• 分子名称: Replicative DNA helicase, DNA replication protein DnaC (2 entities in total)
• 機能のキーワード: helicase, dnab, helicase loader protein, dnac, structural genomics, replication, hydrolase
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 78387.59

構造登録者

Nagata, K.,Okada, A.,Ohtsuka, J.,Ohkuri, T.,Akama, Y.,Sakiyama, Y.,Miyazaki, E.,Horita, S.,Katayama, T.,Ueda, T.,Tanokura, M. (登録日: 2019-09-23, 公開日: 2019-11-20, 最終更新日: 2024-03-27)

主引用文献

Nagata, K.,Okada, A.,Ohtsuka, J.,Ohkuri, T.,Akama, Y.,Sakiyama, Y.,Miyazaki, E.,Horita, S.,Katayama, T.,Ueda, T.,Tanokura, M.
Crystal structure of the complex of the interaction domains of Escherichia coli DnaB helicase and DnaC helicase loader: structural basis implying a distortion-accumulation mechanism for the DnaB ring opening caused by DnaC binding.
J.Biochem., 167:1-14, 2020

PubMed Abstract: Loading the bacterial replicative helicase DnaB onto DNA requires a specific loader protein, DnaC/DnaI, which creates the loading-competent state by opening the DnaB hexameric ring. To understand the molecular mechanism by which DnaC/DnaI opens the DnaB ring, we solved 3.1-Å co-crystal structure of the interaction domains of Escherichia coli DnaB-DnaC. The structure reveals that one N-terminal domain (NTD) of DnaC interacts with both the linker helix of a DnaB molecule and the C-terminal domain (CTD) of the adjacent DnaB molecule by forming a three α-helix bundle, which fixes the relative orientation of the two adjacent DnaB CTDs. The importance of the intermolecular interface in the crystal structure was supported by the mutational data of DnaB and DnaC. Based on the crystal structure and other available information on DnaB-DnaC structures, we constructed a molecular model of the hexameric DnaB CTDs bound by six DnaC NTDs. This model suggested that the binding of a DnaC would cause a distortion in the hexameric ring of DnaB. This distortion of the DnaB ring might accumulate by the binding of up to six DnaC molecules, resulting in the DnaB ring to open.

PubMed: 31665315
DOI: 10.1093/jb/mvz087

実験手法

X-RAY DIFFRACTION (3.1 Å)