6KQS

Crystal Structure of GH136 lacto-N-biosidase from Eubacterium ramulus - selenomethionine derivative

6KQS の概要

• エントリーDOI: 10.2210/pdb6kqs/pdb
• 分子名称: lacto-N-biosidase, beta-D-galactopyranose-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: beta helix, hydrolase
• 由来する生物種: Eubacterium ramulus ATCC 29099
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 77328.15

構造登録者

Yamada, C.,Arakawa, T.,Pichler, M.J.,Abou Hachem, M.,Fushinobu, S. (登録日: 2019-08-18, 公開日: 2020-06-10, 最終更新日: 2024-10-30)

主引用文献

Pichler, M.J.,Yamada, C.,Shuoker, B.,Alvarez-Silva, C.,Gotoh, A.,Leth, M.L.,Schoof, E.,Katoh, T.,Sakanaka, M.,Katayama, T.,Jin, C.,Karlsson, N.G.,Arumugam, M.,Fushinobu, S.,Abou Hachem, M.
Butyrate producing colonic Clostridiales metabolise human milk oligosaccharides and cross feed on mucin via conserved pathways.
Nat Commun, 11:3285-3285, 2020

PubMed Abstract: The early life human gut microbiota exerts life-long health effects on the host, but the mechanisms underpinning its assembly remain elusive. Particularly, the early colonization of Clostridiales from the Roseburia-Eubacterium group, associated with protection from colorectal cancer, immune- and metabolic disorders is enigmatic. Here, we describe catabolic pathways that support the growth of Roseburia and Eubacterium members on distinct human milk oligosaccharides (HMOs). The HMO pathways, which include enzymes with a previously unknown structural fold and specificity, were upregulated together with additional glycan-utilization loci during growth on selected HMOs and in co-cultures with Akkermansia muciniphila on mucin, suggesting an additional role in enabling cross-feeding and access to mucin O-glycans. Analyses of 4599 Roseburia genomes underscored the preponderance and diversity of the HMO utilization loci within the genus. The catabolism of HMOs by butyrate-producing Clostridiales may contribute to the competitiveness of this group during the weaning-triggered maturation of the microbiota.

PubMed: 32620774
DOI: 10.1038/s41467-020-17075-x

実験手法

X-RAY DIFFRACTION (1.4 Å)