6KP3

STRUCTURE OF SENDAI VIRUS Y3/ALIX-BRO1 DOMAIN COMPLEX

6KP3 の概要

• エントリーDOI: 10.2210/pdb6kp3/pdb
• 分子名称: Programmed cell death 6-interacting protein, C' protein (3 entities in total)
• 機能のキーワード: virus budding, viral protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 56362.85

構造登録者

Oda, K.,Matoba, Y.,Sakaguchi, T. (登録日: 2019-08-14, 公開日: 2020-08-19, 最終更新日: 2023-11-22)

主引用文献

Oda, K.,Matoba, Y.,Sugiyama, M.,Sakaguchi, T.
Structural Insight into the Interaction of Sendai Virus C Protein with Alix To Stimulate Viral Budding.
J.Virol., :JVI0081521-JVI0081521, 2021

PubMed Abstract: Sendai virus (SeV), belonging to the genus of the family , harbors an accessory protein, named C protein, which facilitates viral pathogenicity in mice. In addition, the C protein is known to stimulate the budding of virus-like particles by binding to the host ALG-2 interacting protein X (Alix), a component of the endosomal sorting complexes required for transport (ESCRT) machinery. However, small interfering RNA (siRNA)-mediated gene knockdown studies suggested that neither Alix nor C protein is related to SeV budding. In the present study, we determined the crystal structure of a complex comprising the C-terminal half of the C protein (Y3) and the Bro1 domain of Alix at a resolution of 2.2 Å to investigate the role of the complex in SeV budding. The structure revealed that a novel consensus sequence, LXXW, which is conserved among C proteins, is important for Alix binding. SeV possessing a mutated C protein with reduced Alix-binding affinity showed impaired virus production, which correlated with the binding affinity. Infectivity analysis showed a 160-fold reduction at 12 h postinfection compared with nonmutated virus, while C protein competes with CHMP4, one subunit of the ESCRT-III complex, for binding to Alix. All together, these results highlight the critical role of C protein in SeV budding. Human parainfluenza virus type I (hPIV1) is a respiratory pathogen affecting young children, immunocompromised patients, and the elderly, with no available vaccines or antiviral drugs. Sendai virus (SeV), a murine counterpart of hPIV1, has been studied extensively to determine the molecular and biological properties of hPIV1. These viruses possess a multifunctional accessory protein, C protein, which is essential for stimulating viral reproduction, but its role in budding remains controversial. In the present study, the crystal structure of the C-terminal half of the SeV C protein associated with the Bro1 domain of Alix, a component of cell membrane modulating machinery ESCRT, was elucidated. Based on the structure, we designed mutant C proteins with different binding affinities to Alix and showed that the interaction between C and Alix is vital for viral budding. These findings provide new insights into the development of new antiviral drugs against hPIV1.

PubMed: 34287046
DOI: 10.1128/JVI.00815-21

実験手法

X-RAY DIFFRACTION (2.2 Å)