6KO8

Crystal structure of the Cholic acid bound RamR determined with XtaLAB Synergy

6KO8 の概要

• エントリーDOI: 10.2210/pdb6ko8/pdb
• 分子名称: Putative regulatory protein, CHOLIC ACID, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: multisite binding pocket, hth-motif, dna binding protein
• 由来する生物種: Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22548.90

構造登録者

Matsumoto, T.,Nakashima, R.,Yamano, A.,Nishino, K. (登録日: 2019-08-08, 公開日: 2019-10-09, 最終更新日: 2023-11-22)

主引用文献

Matsumoto, T.,Nakashima, R.,Yamano, A.,Nishino, K.
Development of a structure determination method using a multidrug-resistance regulator protein as a framework.
Biochem.Biophys.Res.Commun., 518:402-408, 2019

PubMed Abstract: The structure determination of organic compounds is desirable for the development of medicines, aroma chemicals, and agricultural chemicals. However, the crystallization of organic compounds is often troublesome, because crystallization requires a relatively large quantity of high purity compounds and crystallization trials often need to be performed repetitively using different conditions. Some proteins are known to be able to bind to various organic compounds. The multidrug-resistance regulator protein RamR is one such protein. We have developed a structure determination method for organic compounds using RamR. RamR bound to organic compounds, including one compound that was not a known ligand for RamR, and the structures of the complexes were successfully determined. Because the RamR crystal is hydrophilic, this method may be useful for compounds that cannot be handled by the crystalline sponge method.

PubMed: 31431261
DOI: 10.1016/j.bbrc.2019.08.070

実験手法

X-RAY DIFFRACTION (1.55 Å)