6KNA

Viral Ubiquitin from Autographa californica Nucleo Polyhedrosis Virus

6KNA の概要

• エントリーDOI: 10.2210/pdb6kna/pdb
• 分子名称: Viral ubiquitin (1 entity in total)
• 機能のキーワード: structure from cyana 2.1, viral protein
• 由来する生物種: Autographa californica multiple nucleopolyhedrovirus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8501.80

構造登録者

Negi, H.,Reddy, P.P.,Das, R. (登録日: 2019-08-05, 公開日: 2020-06-17, 最終更新日: 2024-05-15)

主引用文献

Negi, H.,Reddy, P.P.,Vengayil, V.,Patole, C.,Laxman, S.,Das, R.
A novel polyubiquitin chain linkage formed by viral Ubiquitin is resistant to host deubiquitinating enzymes.
Biochem.J., 477:2193-2219, 2020

PubMed Abstract: The Baculoviridae family of viruses encode a viral Ubiquitin (vUb) gene. Though the vUb is homologous to the host eukaryotic Ubiquitin (Ub), its preservation in the viral genome indicates unique functions that are not compensated by the host Ub. We report the structural, biophysical, and biochemical properties of the vUb from Autographa californica multiple nucleo-polyhedrosis virus (AcMNPV). The packing of central helix α1 to the beta-sheet β1-β5 is different between vUb and Ub. Consequently, its stability is lower compared with Ub. However, the surface properties, ubiquitination activity, and the interaction with Ubiquitin-binding domains are similar between vUb and Ub. Interestingly, vUb forms atypical polyubiquitin chain linked by lysine at the 54th position (K54), and the deubiquitinating enzymes are ineffective against the K54-linked polyubiquitin chains. We propose that the modification of host/viral proteins with the K54-linked chains is an effective way selected by the virus to protect the vUb signal from host DeUbiquitinases.

PubMed: 32478812
DOI: 10.1042/BCJ20200289

実験手法

SOLUTION NMR