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6KM8

Crystal Structure of Momordica charantia 7S globulin

Summary for 6KM8
Entry DOI10.2210/pdb6km8/pdb
Descriptor7S globulin, COPPER (II) ION, ACETATE ION, ... (4 entities in total)
Functional Keywords7s globulin bi-cupin fold, glycosylated, copper-ion, plant protein
Biological sourceMomordica charantia
Total number of polymer chains1
Total formula weight43118.99
Authors
Kesari, P.,Pratap, S.,Dhankhar, P.,Dalal, V.,Kumar, P. (deposition date: 2019-07-31, release date: 2020-02-12, Last modification date: 2023-11-22)
Primary citationKesari, P.,Pratap, S.,Dhankhar, P.,Dalal, V.,Mishra, M.,Singh, P.K.,Chauhan, H.,Kumar, P.
Structural characterization and in-silico analysis of Momordica charantia 7S globulin for stability and ACE inhibition.
Sci Rep, 10:1160-1160, 2020
Cited by
PubMed Abstract: Momordica charantia (Mc) seeds are widely used edible crop with high nutritional quality. The food and pharmaceutical industries use it as a natural anti-oxygenic agent. Herein, a ~52 kDa protein, which is a major part of seed proteome has been purified, biochemically characterized and structure has been determined. MALDI-ESI-MS identified peptide fragments and contig-deduced sequence suggested the protein to be homologous to 7S globulins. The crystal structure shows that protein has a bicupin fold similar to 7S globulins and the electron density for a copper and acetate ligand were observed in the C-terminal barrel domain. In silico study reveals that a tripeptide (VFK) from Mc7S possess a higher binding affinity for angiotensin converting enzyme (ACE) than already reported drug Lisinopril (LPR). The protein is a glycoprotein and highly stable under varying thermal and pH conditions due to its secondary structures. The DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) assay showed the protein to have an anti-oxygenic nature and can aid in scavenging free radical from sample. The protein can assist to enhance the nutritional and functional value of food by acting as a food antioxidant. Further, characterization of Mc7S required which might add in importance of Mc7S as antioxidant, anti-diabetic and anti-hypertensive.
PubMed: 31980708
DOI: 10.1038/s41598-020-58138-9
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (3.099 Å)
Structure validation

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