6KM8
Crystal Structure of Momordica charantia 7S globulin
Summary for 6KM8
Entry DOI | 10.2210/pdb6km8/pdb |
Descriptor | 7S globulin, COPPER (II) ION, ACETATE ION, ... (4 entities in total) |
Functional Keywords | 7s globulin bi-cupin fold, glycosylated, copper-ion, plant protein |
Biological source | Momordica charantia |
Total number of polymer chains | 1 |
Total formula weight | 43118.99 |
Authors | Kesari, P.,Pratap, S.,Dhankhar, P.,Dalal, V.,Kumar, P. (deposition date: 2019-07-31, release date: 2020-02-12, Last modification date: 2023-11-22) |
Primary citation | Kesari, P.,Pratap, S.,Dhankhar, P.,Dalal, V.,Mishra, M.,Singh, P.K.,Chauhan, H.,Kumar, P. Structural characterization and in-silico analysis of Momordica charantia 7S globulin for stability and ACE inhibition. Sci Rep, 10:1160-1160, 2020 Cited by PubMed Abstract: Momordica charantia (Mc) seeds are widely used edible crop with high nutritional quality. The food and pharmaceutical industries use it as a natural anti-oxygenic agent. Herein, a ~52 kDa protein, which is a major part of seed proteome has been purified, biochemically characterized and structure has been determined. MALDI-ESI-MS identified peptide fragments and contig-deduced sequence suggested the protein to be homologous to 7S globulins. The crystal structure shows that protein has a bicupin fold similar to 7S globulins and the electron density for a copper and acetate ligand were observed in the C-terminal barrel domain. In silico study reveals that a tripeptide (VFK) from Mc7S possess a higher binding affinity for angiotensin converting enzyme (ACE) than already reported drug Lisinopril (LPR). The protein is a glycoprotein and highly stable under varying thermal and pH conditions due to its secondary structures. The DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) assay showed the protein to have an anti-oxygenic nature and can aid in scavenging free radical from sample. The protein can assist to enhance the nutritional and functional value of food by acting as a food antioxidant. Further, characterization of Mc7S required which might add in importance of Mc7S as antioxidant, anti-diabetic and anti-hypertensive. PubMed: 31980708DOI: 10.1038/s41598-020-58138-9 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (3.099 Å) |
Structure validation
Download full validation report