6KLS

Hyperthermophilic respiratory Complex III

6KLS の概要

• エントリーDOI: 10.2210/pdb6kls/pdb
• EMDBエントリー: 0716
• 分子名称: Rieske-I iron sulfur protein, cytochrome b subunit, Cytochrome c, ... (8 entities in total)
• 機能のキーワード: respiratory chain, complex iii, hyperthermophilic mechanism, oxidoreductase
• 由来する生物種: Aquifex aeolicus (strain VF5); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 198446.95

構造登録者

Fei, S.,Hartmut, M.,Yun, Z.,Guohong, P.,Guoliang, Z.,Hui, Z.,Shuangbo, Z.,Xiaoyun, P.,Yan, Z. (登録日: 2019-07-30, 公開日: 2020-05-13, 最終更新日: 2025-09-17)

主引用文献

Zhu, G.,Zeng, H.,Zhang, S.,Juli, J.,Pang, X.,Hoffmann, J.,Zhang, Y.,Morgner, N.,Zhu, Y.,Peng, G.,Michel, H.,Sun, F.
A 3.3 angstrom -Resolution Structure of Hyperthermophilic Respiratory Complex III Reveals the Mechanism of Its Thermal Stability.
Angew.Chem.Int.Ed.Engl., 59:343-351, 2020

PubMed Abstract: Respiratory chain complexes convert energy by coupling electron flow to transmembrane proton translocation. Owing to a lack of atomic structures of cytochrome bc complex (Complex III) from thermophilic bacteria, little is known about the adaptations of this macromolecular machine to hyperthermophilic environments. In this study, we purified the cytochrome bc complex of Aquifex aeolicus, one of the most extreme thermophilic bacteria known, and determined its structure with and without an inhibitor at 3.3 Å resolution. Several residues unique for thermophilic bacteria were detected that provide additional stabilization for the structure. An extra transmembrane helix at the N-terminus of cyt. c was found to greatly enhance the interaction between cyt. b and cyt. c , and to bind a phospholipid molecule to stabilize the complex in the membrane. These results provide the structural basis for the hyperstability of the cytochrome bc complex in an extreme thermal environment.

PubMed: 31778296
DOI: 10.1002/anie.201911554

実験手法

ELECTRON MICROSCOPY (3.3 Å)