6KLN

F-actin of cardiac thin filament in high-calcium state

6KLN の概要

• エントリーDOI: 10.2210/pdb6kln/pdb
• EMDBエントリー: 0711 0712
• 分子名称: Actin, alpha skeletal muscle, MAGNESIUM ION, ADENOSINE-5'-DIPHOSPHATE (3 entities in total)
• 機能のキーワード: cardiac thin filament, contractile protein
• 由来する生物種: Mus musculus (Mouse)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 169308.56

構造登録者

Oda, T.,Yanagisawa, H.,Wakabayashi, T. (登録日: 2019-07-30, 公開日: 2020-01-15, 最終更新日: 2020-03-11)

主引用文献

Oda, T.,Yanagisawa, H.,Wakabayashi, T.
Cryo-EM structures of cardiac thin filaments reveal the 3D architecture of troponin.
J.Struct.Biol., 209:107450-107450, 2020

PubMed Abstract: Troponin is an essential component of striated muscle and it regulates the sliding of actomyosin system in a calcium-dependent manner. Despite its importance, the structure of troponin has been elusive due to its high structural heterogeneity. In this study, we analyzed the 3D structures of murine cardiac thin filaments using a cryo-electron microscope equipped with a Volta phase plate (VPP). Contrast enhancement by a VPP enabled us to reconstruct the entire repeat of the thin filament. We determined the orientation of troponin relative to F-actin and tropomyosin, and characterized the interactions between troponin and tropomyosin. This study provides a structural basis for understanding the molecular mechanism of actomyosin system.

PubMed: 31954841
DOI: 10.1016/j.jsb.2020.107450

実験手法

ELECTRON MICROSCOPY (3.4 Å)