6KII

photolyase from Arthrospira platensis

Summary for 6KII

• Entry DOI: 10.2210/pdb6kii/pdb
• Descriptor: Deoxyribopyrimidine photolyase, FLAVIN-ADENINE DINUCLEOTIDE, 5,10-METHENYL-6,7,8-TRIHYDROFOLIC ACID, ... (5 entities in total)
• Functional Keywords: photolyase, dna binding protein, lyase
• Biological source: Arthrospira platensis
• Total number of polymer chains: 1
• Total formula weight: 58523.81

Authors

Yan, H.,Zhu, K. (deposition date: 2019-07-18, release date: 2020-07-22, Last modification date: 2023-11-22)

Primary citation

Yan, H.,Zhu, K.,Teng, M.,Li, X.
A newly identified photolyase from Arthrospira platensis possesses a unique methenyltetrahydrofolate chromophore-binding pattern.
Febs Lett., 594:740-750, 2020

PubMed Abstract: Cyclobutane pyrimidine dimers (CPD), as a common DNA damage caused by UV radiation, often lead to skin cancer. Here, we identified a photolyase from the alga Arthrospira platensis (designated as Ap-phr), which has been regarded as a safe organism for humans for centuries, that can efficiently repair CPD lesions in ssDNA and dsDNA in vitro. The 1.6 Å resolution crystal structure of Ap-phr revealed that it possesses a unique methenyltetrahydrofolate chromophore-binding pattern with high energy transfer efficiency. Our study of Ap-phr highlights its potential use in cosmetic, industrial and aesthetic medicine applications.

PubMed: 31675429
DOI: 10.1002/1873-3468.13657

Experimental method

X-RAY DIFFRACTION (1.6 Å)