6KIF

Structure of cyanobacterial photosystem I-IsiA-flavodoxin supercomplex

これはPDB形式変換不可エントリーです。

6KIF の概要

• エントリーDOI: 10.2210/pdb6kif/pdb
• EMDBエントリー: 9994
• 分子名称: Photosystem I P700 chlorophyll a apoprotein A1, Photosystem I reaction center subunit XI, PsaM, ... (23 entities in total)
• 機能のキーワード: photosystem, antenna, chlorophyll-binding protein, membrane protein, photosynthesis, iron stress-induced protein a, flavodoxin
• 由来する生物種: Synechococcus elongatus (strain PCC 7942); 詳細
• タンパク質・核酸の鎖数: 54
• 化学式量合計: 2159108.06

構造登録者

Cao, P.,Cao, D.F.,Si, L.,Su, X.D.,Chang, W.R.,Liu, Z.F.,Zhang, X.Z.,Li, M. (登録日: 2019-07-18, 公開日: 2020-02-12, 最終更新日: 2024-10-23)

主引用文献

Cao, P.,Cao, D.,Si, L.,Su, X.,Tian, L.,Chang, W.,Liu, Z.,Zhang, X.,Li, M.
Structural basis for energy and electron transfer of the photosystem I-IsiA-flavodoxin supercomplex.
Nat.Plants, 6:167-176, 2020

PubMed Abstract: Under iron-deficiency stress, which occurs frequently in natural aquatic environments, cyanobacteria reduce the amount of iron-enriched proteins, including photosystem I (PSI) and ferredoxin (Fd), and upregulate the expression of iron-stress-induced proteins A and B (IsiA and flavodoxin (Fld)). Multiple IsiAs function as the peripheral antennae that encircle the PSI core, whereas Fld replaces Fd as the electron receptor of PSI. Here, we report the structures of the PSI-IsiA-Fld and PSI-IsiA supercomplexes from Synechococcus sp. PCC 7942, revealing features that are different from the previously reported PSI structures, and a sophisticated pigment network that involves previously unobserved pigment molecules. Spectroscopic results demonstrated that IsiAs are efficient light harvesters for PSI. Three Flds bind symmetrically to the trimeric PSI core-we reveal the detailed interaction and the electron transport path between PSI and Fld. Our results provide a structural basis for understanding the mechanisms of light harvesting, energy transfer and electron transport of cyanobacterial PSI under stressed conditions.

PubMed: 32042157
DOI: 10.1038/s41477-020-0593-7

実験手法

ELECTRON MICROSCOPY (3.3 Å)