6KHI

Supercomplex for cylic electron transport in cyanobacteria

Summary for 6KHI

• Entry DOI: 10.2210/pdb6khi/pdb
• EMDB information: 9989 9990
• Descriptor: NAD(P)H-quinone oxidoreductase subunit 1, NAD(P)H-quinone oxidoreductase subunit J, NAD(P)H-quinone oxidoreductase subunit K, ... (27 entities in total)
• Functional Keywords: cylic electron transport, photosynthetic ndh complex, cyanobacteria, electron transport
• Biological source: Thermosynechococcus elongatus BP-1; More
• Total number of polymer chains: 20
• Total formula weight: 493452.31

Authors

Pan, X.,Cao, D.,Xie, F.,Zhang, X.,Li, M. (deposition date: 2019-07-15, release date: 2020-02-12, Last modification date: 2024-03-27)

Primary citation

Pan, X.,Cao, D.,Xie, F.,Xu, F.,Su, X.,Mi, H.,Zhang, X.,Li, M.
Structural basis for electron transport mechanism of complex I-like photosynthetic NAD(P)H dehydrogenase.
Nat Commun, 11:610-610, 2020

PubMed Abstract: NAD(P)H dehydrogenase-like (NDH) complex NDH-1L of cyanobacteria plays a crucial role in cyclic electron flow (CEF) around photosystem I and respiration processes. NDH-1L couples the electron transport from ferredoxin (Fd) to plastoquinone (PQ) and proton pumping from cytoplasm to the lumen that drives the ATP production. NDH-1L-dependent CEF increases the ATP/NADPH ratio, and is therefore pivotal for oxygenic phototrophs to function under stress. Here we report two structures of NDH-1L from Thermosynechococcus elongatus BP-1, in complex with one Fd and an endogenous PQ, respectively. Our structures represent the complete model of cyanobacterial NDH-1L, revealing the binding manner of NDH-1L with Fd and PQ, as well as the structural elements crucial for proper functioning of the NDH-1L complex. Together, our data provides deep insights into the electron transport from Fd to PQ, and its coupling with proton translocation in NDH-1L.

PubMed: 32001694
DOI: 10.1038/s41467-020-14456-0

Experimental method

ELECTRON MICROSCOPY (3 Å)