6KH4

Design and crystal structure of protein MOFs with ferritin nanocages as linkers and nickel clusters as nodes

6KH4 の概要

• エントリーDOI: 10.2210/pdb6kh4/pdb
• 分子名称: Ferritin, FE (III) ION, NICKEL (II) ION, ... (4 entities in total)
• 機能のキーワード: metal binding protein
• 由来する生物種: Penaeus japonicus (Kuruma prawn)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 19766.75

構造登録者

Gu, C.,Chen, H.,Wang, Y.,Zhang, T.,Wang, H.,Zhao, G. (登録日: 2019-07-12, 公開日: 2020-01-29, 最終更新日: 2023-11-22)

主引用文献

Gu, C.,Chen, H.,Wang, Y.,Zhang, T.,Wang, H.,Zhao, G.
Structural Insight into Binary Protein Metal-Organic Frameworks with Ferritin Nanocages as Linkers and Nickel Clusters as Nodes.
Chemistry, 26:3016-3021, 2020

PubMed Abstract: Metal-organic frameworks (MOFs) hold great promise for numerous applications. However, proteins, carriers of biological functions in living systems, have not yet been fully explored as building blocks for the construction of MOFs. This work presents a strategy for the fabrication of binary MOFs. Considering octahedral ferritin symmetry, four His (His-His) motifs were first incorporated into the exterior surface of a ferritin nanocage near each C channel, yielding protein linkers with multiple metal-binding sites (bisH-SF). Secondly, by adding nickel ions to bisH-SF solutions triggers the self-assembly of ferritin nanocages into a porous 3D crystalline MOF with designed protein lattice, where two adjacent ferritin molecules along the C symmetry axes are bridged by four dinuclear or tetranuclear nickel clusters depending on Ni concentration. This work provides a simple approach for precise control over a binary protein-metal crystalline framework, and the resulting MOFs exhibited inherent ferroxidase activity and peroxidase-like catalytic activity.

PubMed: 31820500
DOI: 10.1002/chem.201905315

実験手法

X-RAY DIFFRACTION (2.302 Å)