6KF9

Cryo-EM structure of Thermococcus kodakarensis RNA polymerase

6KF9 の概要

• エントリーDOI: 10.2210/pdb6kf9/pdb
• EMDBエントリー: 9960 9962 9970
• 分子名称: DNA-directed RNA polymerase subunit, DNA-directed RNA polymerase subunit N, DNA-directed RNA polymerase subunit P, ... (16 entities in total)
• 機能のキーワード: apo-rna polymerase, transcription
• 由来する生物種: Thermococcus kodakarensis KOD1; 詳細
• タンパク質・核酸の鎖数: 14
• 化学式量合計: 415639.43

構造登録者

Jun, S.-H.,Hyun, J.,Jeong, H.,Cha, J.S.,Kim, H.,Bartlett, M.S.,Cho, H.-S.,Murakami, K.S. (登録日: 2019-07-07, 公開日: 2020-07-01, 最終更新日: 2024-05-29)

主引用文献

Jun, S.H.,Hyun, J.,Cha, J.S.,Kim, H.,Bartlett, M.S.,Cho, H.S.,Murakami, K.S.
Direct binding of TFE alpha opens DNA binding cleft of RNA polymerase.
Nat Commun, 11:6123-6123, 2020

PubMed Abstract: Opening of the DNA binding cleft of cellular RNA polymerase (RNAP) is necessary for transcription initiation but the underlying molecular mechanism is not known. Here, we report on the cryo-electron microscopy structures of the RNAP, RNAP-TFEα binary, and RNAP-TFEα-promoter DNA ternary complexes from archaea, Thermococcus kodakarensis (Tko). The structures reveal that TFEα bridges the RNAP clamp and stalk domains to open the DNA binding cleft. Positioning of promoter DNA into the cleft closes it while maintaining the TFEα interactions with the RNAP mobile modules. The structures and photo-crosslinking results also suggest that the conserved aromatic residue in the extended winged-helix domain of TFEα interacts with promoter DNA to stabilize the transcription bubble. This study provides a structural basis for the functions of TFEα and elucidates the mechanism by which the DNA binding cleft is opened during transcription initiation in the stalk-containing RNAPs, including archaeal and eukaryotic RNAPs.

PubMed: 33257704
DOI: 10.1038/s41467-020-19998-x

実験手法

ELECTRON MICROSCOPY (3.79 Å)