6K8G

H/D exchanged Hen egg-white lysozyme

Summary for 6K8G

• Entry DOI: 10.2210/pdb6k8g/pdb
• Descriptor: Lysozyme C, CHLORIDE ION, SODIUM ION, ... (4 entities in total)
• Functional Keywords: h/d exchanged protein, hydrolase
• Biological source: Gallus gallus (Chicken)
• Total number of polymer chains: 1
• Total formula weight: 14389.60

Authors

Kita, A.,Morimoto, Y. (deposition date: 2019-06-11, release date: 2020-06-17, Last modification date: 2024-04-03)

Primary citation

Kita, A.,Morimoto, Y.
Hydrogen/deuterium exchange behavior in tetragonal hen egg-white lysozyme crystals affected by solution state.
J.Appl.Crystallogr., 53:837-840, 2020

PubMed Abstract: Neutron diffraction studies of hydrogen/deuterium-exchanged hen egg-white lysozyme were performed by a joint X-ray and neutron refinement to elucidate the hydrogen/deuterium exchange behavior. Large crystals for neutron work, consisting of molecules that were exchanged before crystallization, were obtained by repeatedly adding protein solution to the crystal batch using deuterated precipitant reagent. There are differences in hydrogen/deuterium exchange behavior compared with previous crystallographic or NMR studies, which could be due to intermolecular interactions in the crystal or to different lengths of exchange period.

PubMed: 32684898
DOI: 10.1107/S1600576720005488

Experimental method

NEUTRON DIFFRACTION (2 Å)
X-RAY DIFFRACTION (2 Å)