6K8G

H/D exchanged Hen egg-white lysozyme

6K8G の概要

• エントリーDOI: 10.2210/pdb6k8g/pdb
• 分子名称: Lysozyme C, CHLORIDE ION, SODIUM ION, ... (4 entities in total)
• 機能のキーワード: h/d exchanged protein, hydrolase
• 由来する生物種: Gallus gallus (Chicken)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14389.60

構造登録者

Kita, A.,Morimoto, Y. (登録日: 2019-06-11, 公開日: 2020-06-17, 最終更新日: 2024-11-20)

主引用文献

Kita, A.,Morimoto, Y.
Hydrogen/deuterium exchange behavior in tetragonal hen egg-white lysozyme crystals affected by solution state.
J.Appl.Crystallogr., 53:837-840, 2020

PubMed Abstract: Neutron diffraction studies of hydrogen/deuterium-exchanged hen egg-white lysozyme were performed by a joint X-ray and neutron refinement to elucidate the hydrogen/deuterium exchange behavior. Large crystals for neutron work, consisting of molecules that were exchanged before crystallization, were obtained by repeatedly adding protein solution to the crystal batch using deuterated precipitant reagent. There are differences in hydrogen/deuterium exchange behavior compared with previous crystallographic or NMR studies, which could be due to intermolecular interactions in the crystal or to different lengths of exchange period.

PubMed: 32684898
DOI: 10.1107/S1600576720005488

実験手法

NEUTRON DIFFRACTION (2 Å)
X-RAY DIFFRACTION (2 Å)