6K5V

Structure of CSY4 Apo-form

6K5V の概要

• エントリーDOI: 10.2210/pdb6k5v/pdb
• 分子名称: Citrate synthase 4, mitochondrial, CHLORIDE ION (3 entities in total)
• 機能のキーワード: citrate synthase, mitochondrial, plant, csy4, transferase
• 由来する生物種: Arabidopsis thaliana (Mouse-ear cress)
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 305606.51

構造登録者

Nishio, K.,Mizushima, T. (登録日: 2019-05-31, 公開日: 2020-03-18, 最終更新日: 2023-11-22)

主引用文献

Nishio, K.,Mizushima, T.
Structural and biochemical characterization of mitochondrial citrate synthase 4 from Arabidopsis thaliana.
Acta Crystallogr.,Sect.F, 76:109-115, 2020

PubMed Abstract: Citrate synthase (CS) catalyzes the conversion of oxaloacetate and acetyl coenzyme A into citrate and coenzyme A in the mitochondrial tricarboxylic acid (TCA) cycle. In plants, mitochondrial metabolism, including the TCA cycle, occurs in interaction with photosynthetic metabolism. The controlled regulation of several enzymes in the TCA cycle, such as CS, is important in plants. Here, the first crystal structure of a plant mitochondrial CS, CSY4 from Arabidopsis thaliana (AtCSY4), has been determined. Structural comparison of AtCSY4 with mitochondrial CSs revealed a high level of similarity. Inhibition analysis showed a similar manner of inhibition as in mitochondrial CSs. The effect of oxidation on one of a pair of cysteine residues in AtCSY4 was speculated upon based on the folded structure.

PubMed: 32133996
DOI: 10.1107/S2053230X20001521

実験手法

X-RAY DIFFRACTION (2.69 Å)