6K2P

Crystal structure of proteinase K from Engyodontium album

6K2P の概要

• エントリーDOI: 10.2210/pdb6k2p/pdb
• 分子名称: Proteinase K, CALCIUM ION, NITRATE ION, ... (4 entities in total)
• 機能のキーワード: xfel, sfx, hydrolase
• 由来する生物種: Parengyodontium album (Tritirachium album,Engyodontium album)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 29100.95

構造登録者

Sugahara, M.,Motomura, K.,Numata, K. (登録日: 2019-05-15, 公開日: 2020-05-20, 最終更新日: 2025-03-05)

主引用文献

Sugahara, M.,Motomura, K.,Suzuki, M.,Masuda, T.,Joti, Y.,Numata, K.,Tono, K.,Yabashi, M.,Ishikawa, T.
Viscosity-adjustable grease matrices for serial nanocrystallography.
Sci Rep, 10:1371-1371, 2020

PubMed Abstract: Serial femtosecond crystallography (SFX) has enabled determination of room temperature structures of proteins with minimum radiation damage. A highly viscous grease matrix acting as a crystal carrier for serial sample loading at a low flow rate of ~0.5 μl min was introduced into the beam path of X-ray free-electron laser. This matrix makes it possible to determine the protein structure with a sample consumption of less than 1 mg of the protein. The viscosity of the matrix is an important factor in maintaining a continuous and stable sample column from a nozzle of a high viscosity micro-extrusion injector for serial sample loading. Using conventional commercial grease (an oil-based, viscous agent) with insufficient control of viscosity in a matrix often gives an unexpectedly low viscosity, providing an unstable sample stream, with effects such as curling of the stream. Adjustment of the grease viscosity is extremely difficult since the commercial grease contains unknown compounds, which may act as unexpected inhibitors of proteins. This study introduces two novel grease matrix carriers comprising known compounds with a viscosity higher than that of conventional greases, to determine the proteinase K structure from nano-/microcrystals.

PubMed: 31992735
DOI: 10.1038/s41598-020-57675-7

実験手法

X-RAY DIFFRACTION (1.6 Å)