6JXU

SUMO1 bound to SLS4-SIM peptide from ICP0

6JXU の概要

• エントリーDOI: 10.2210/pdb6jxu/pdb
• 分子名称: Small ubiquitin-related modifier, viral protein (2 entities in total)
• 機能のキーワード: sumoylation, protein binding-peptide complex, protein binding/peptide
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 12902.43

構造登録者

Hembram, D.S.S.,Negi, H.,Shet, D.,Das, R. (登録日: 2019-04-25, 公開日: 2020-02-05, 最終更新日: 2024-05-15)

主引用文献

Hembram, D.S.S.,Negi, H.,Biswas, P.,Tripathi, V.,Bhushan, L.,Shet, D.,Kumar, V.,Das, R.
The Viral SUMO-Targeted Ubiquitin Ligase ICP0 is Phosphorylated and Activated by Host Kinase Chk2.
J.Mol.Biol., 432:1952-1977, 2020

PubMed Abstract: When the herpes simplex virus (HSV) genome enters the nucleus for replication and transcription, phase-segregated nuclear protein bodies called Promyelocytic leukemia protein nuclear bodies (PML NBs) colocalize with the genome and repress it. HSV encodes a small ubiquitin-like modifier (SUMO)-targeted ubiquitin ligase (STUbL) infected cell polypeptide 0 (ICP0) that degrades PML NBs to alleviate the repression. The molecular details of the mechanism used by ICP0 to target PML NBs are unclear. Here, we identify a bona fide SUMO-interacting motif in ICP0 (SIM-like sequence [SLS] 4) that is essential and sufficient to target SUMOylated proteins in PML NBs such as the PML and Sp100. We shown that phosphorylation of SLS4 creates new salt bridges between SUMO and SLS4, increases the SUMO/SLS4 affinity, and switches ICP0 into a potent STUbL. HSV activates the Ataxia-telangiectasia-mutated kinase-Checkpoint kinase 2 (ATM-Chk2) pathway to regulate the cell cycle of the host. We report that the activated Chk2 also phosphorylates ICP0 at SLS4 and enhances its STUbL activity. Our results uncover that a viral STUbL counters antiviral response by exploiting an unprecedented cross-talk of three post-translational modifications: ubiquitination, SUMOylation, and phosphorylation.

PubMed: 32001251
DOI: 10.1016/j.jmb.2020.01.021

実験手法

SOLUTION NMR