6JQY

Crystal structure of N-terminal domain of VapB46 antitoxin from Mycobacterium tuberculosis

6JQY の概要

• エントリーDOI: 10.2210/pdb6jqy/pdb
• 分子名称: Antitoxin VapB46, ACETATE ION (3 entities in total)
• 機能のキーワード: phd/yefm fold, dna binding, virulence associated protein, antitoxin, dna binding protein
• 由来する生物種: Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 19408.28

構造登録者

Roy, M.,Singh, B.K.,Bhattacharyya, S.,Das, A.K. (登録日: 2019-04-02, 公開日: 2020-04-08, 最終更新日: 2025-10-29)

主引用文献

Roy, M.,Saha, R.,Singh, B.,Yadav, V.K.,Bhattacharyya, S.,De, S.,Das, A.K.
Crystal structure of N-terminal VapB46 antitoxin and interaction analysis of its cognate partners from molecular dynamics simulation.
Biochem.Biophys.Res.Commun., 783:152592-152592, 2025

PubMed Abstract: Toxin-antitoxin (TA) systems are important for persister cell formation in Mycobacterium tuberculosis (Mtb), which aids their survival during stress condition. Antitoxins regulate the TA systems by binding to the promoter-operator region. Among fifty VapBC TA systems in Mtb, few have been characterized, while remaining needs characterisation to understand the VapBC TA mechanism. Here, we report the crystal structure of the N-terminal domain of VapB46 antitoxin at a resolution of 1.64 Å. The protein has a DNA-binding domain that resembles the N-terminal domain of Phd antitoxin with a βααββ fold. Simulation study revealed that VapB46 binds as a dimer at two promoter/operator DNA sites, and tetrameric VapB46 binds to VapC46 toxin via its C-terminal domain. The analysis of the structure and simulation study provides detailed insights into the VapBC46 TA system, paving the way for future drug development targeting this system.

PubMed: 40934556
DOI: 10.1016/j.bbrc.2025.152592

実験手法

X-RAY DIFFRACTION (1.643 Å)