6JPK

Crystal structure of S. pombe aspartate aminotransferase

6JPK の概要

• エントリーDOI: 10.2210/pdb6jpk/pdb
• 分子名称: Aspartate aminotransferase, cytoplasmic, PHOSPHATE ION, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: amino acid, aspartate, aminotransferase, plp-dependent enzyme, pyridoxal 5'-phosphate dependent enzyme, vitamin b6 dependent enzyme, transferase
• 由来する生物種: Schizosaccharomyces pombe 972h- (Fission yeast)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 94936.93

構造登録者

Jin, H.,Chang, J.H. (登録日: 2019-03-27, 公開日: 2019-09-11, 最終更新日: 2023-11-22)

主引用文献

Jeong, S.Y.,Jin, H.,Chang, J.H.
Crystal structure of L-aspartate aminotransferase from Schizosaccharomyces pombe.
Plos One, 14:e0221975-e0221975, 2019

PubMed Abstract: L-aspartate aminotransferase is a pyridoxal 5'-phosphate-dependent transaminase that catalyzes reversible transfer of an α-amino group from aspartate to α-ketoglutarate or from glutamate to oxaloacetate. L-aspartate aminotransferase not only mediates amino acid and carbohydrate metabolism but also regulates the cellular level of amino acids by catalyzing amino acid degradation and biosynthesis. To expand our structural information, we determined the crystal structure of L-aspartate aminotransferase from Schizosaccharomyces pombe at 2.1 Å resolution. A structural comparison between two yeast L-aspartate aminotransferases revealed conserved enzymatic mechanism mediated by the open-closed conformational change. Compared with higher eukaryotic species, L-aspartate aminotransferases showed distinguishable inter-subunit interaction between the N-terminal arm and a large domain of the opposite subunit. Interestingly, structural homology search showed varied conformation of the N-terminal arm among 71 structures of the family. Therefore, we classified pyridoxal 5'-phosphate-dependent enzymes into eight subclasses based on the structural feature of N-terminal arms. In addition, structure and sequence comparisons showed strong relationships among the eight subclasses. Our results may provide insights into structure-based evolutionary aspects of pyridoxal 5'-phosphate-dependent enzymes.

PubMed: 31465495
DOI: 10.1371/journal.pone.0221975

実験手法

X-RAY DIFFRACTION (2.102 Å)