6JOX

triosephosphate isomerase-scylla paramamosain

6JOX の概要

• エントリーDOI: 10.2210/pdb6jox/pdb
• 分子名称: Triosephosphate isomerase (2 entities in total)
• 機能のキーワード: triosephosphate isomerase, allergen, blue crab, food allergy, isomerase
• 由来する生物種: Scylla paramamosain (Mud crab)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 58614.91

構造登録者

Xia, F.,Jin, T. (登録日: 2019-03-25, 公開日: 2019-12-04, 最終更新日: 2023-11-22)

主引用文献

Xia, F.,Li, M.S.,Liu, Q.M.,Liu, M.,Yang, Y.,Cao, M.J.,Chen, G.X.,Jin, T.,Liu, G.M.
Crystal Structure Analysis and Conformational Epitope Mutation of Triosephosphate Isomerase, a Mud Crab Allergen.
J.Agric.Food Chem., 67:12918-12926, 2019

PubMed Abstract: The triosephosphate isomerase (TIM), Scy p 8, is a crab allergen and shows cross-reactivity in the shellfish. Here, recombinant Scy p 8 was expressed, and its crystal structure was determined at a resolution of 1.8 Å. The three-dimensional structure of Scy p 8 is primarily composed of a (β/α)-barrel motif prototype. Additionally, Scy p 8 showed cross-reactivity with high sequential and secondary structural identity among TIMs from shellfish species. The site-directed mutagenesis of critical amino acids of conformational epitopes was carried out, and the mutants of Trp 168 and Lys 237 to Ala reduced immunoglobulin E (IgE)-binding activity by approximately 30%, compared with wild-type TIM in an inhibition ELISA; however, it still induced basophil activation despite the interpatient variability between patients. These results can help to provide an accurate template for the analysis of the IgE binding and establish meaningful relationships between structure and allergenicity.

PubMed: 31668066
DOI: 10.1021/acs.jafc.9b05279

実験手法

X-RAY DIFFRACTION (1.803 Å)