6JNT

Catalase structure determined by eEFD (dataset 1)

6JNT の概要

• エントリーDOI: 10.2210/pdb6jnt/pdb
• 関連するPDBエントリー: 3NWL
• 分子名称: Catalase, PROTOPORPHYRIN IX CONTAINING FE, NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, ... (4 entities in total)
• 機能のキーワード: electron 3d crystallography, eefd, energy filter, parallem, cryo arm, oxidoreductase
• 由来する生物種: Bos taurus (Bovine)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 245444.27

構造登録者

Yonekura, K.,Maki-Yonekura, S. (登録日: 2019-03-18, 公開日: 2019-04-24, 最終更新日: 2024-03-27)

主引用文献

Yonekura, K.,Ishikawa, T.,Maki-Yonekura, S.
A new cryo-EM system for electron 3D crystallography by eEFD.
J.Struct.Biol., 206:243-253, 2019

PubMed Abstract: A new cryo-EM system has been developed and investigated for use in protein electron 3D crystallography. The system provides parallel illumination of a coherent 300 kV electron beam to a sample, filters out energy-loss electrons through the sample with an in-column energy filter, and allows rotational data collection on a fast camera. It also possesses motorized cryo-sample loading and automated liquid-nitrogen filling for cooling of multiple samples. To facilitate its use, we developed GUI programs for efficient operation and accurate structure analysis. Here we report on the performance of the system and first results for thin 3D crystals of the protein complexes, catalase and membrane protein complex ExbBD. Data quality is remarkably improved with this approach, which we name eEFD (electron energy-filtered diffraction of 3D crystals), compared with those collected at 200 kV without energy filtration. Key advances include precise control of the microscope and recordings of lens fluctuations, which the programs process and respond to. We also discuss the merits of higher-energy electrons and filtration of energy-loss electrons in electron 3D crystallography.

PubMed: 30928615
DOI: 10.1016/j.jsb.2019.03.009

実験手法

ELECTRON CRYSTALLOGRAPHY (3 Å)