6JHA

Crystal structure of NADPH bound AerF from Microcystis aeruginosa

6JHA の概要

• エントリーDOI: 10.2210/pdb6jha/pdb
• 分子名称: Short chain dehydrogenase family protein, NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, TRIETHYLENE GLYCOL, ... (4 entities in total)
• 機能のキーワード: reductase, biosynthetic protein
• 由来する生物種: Microcystis aeruginosa DIANCHI905
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 62474.32

構造登録者

Qiu, X. (登録日: 2019-02-17, 公開日: 2019-11-27, 最終更新日: 2023-11-22)

主引用文献

Qiu, X.,Wei, Y.,Zhu, W.,Fu, J.,Duan, X.,Jin, H.,Zhu, P.,Zhou, C.,Yan, X.
Structural and functional investigation of AerF, a NADPH-dependent alkenal double bond reductase participating in the biosynthesis of Choi moiety of aeruginosin
J.Struct.Biol., 2019

PubMed Abstract: The 2-carboxy-6-hydroxyoctahydroindole (Choi) moiety is an essential residue for the antithrombotic activities of aeruginosins, which are a class of cyanobacterial derived bioactive linear tetrapeptides. Biosynthetic pathway of Choi is still elusive. AerF was suggested to be involved in the biosynthesis of Choi, and can be assigned to the short-chain dehydrogenase/reductase (SDR) superfamily. However, both the exact role and the catalytic mechanism of AerF have not been elucidated. In this study, functional and mechanistic analyses of AerF from Microcystis aeruginosa were performed. Observation of enzymatic assay demonstrates that AerF is a NADPH-dependent alkenal double bond reductase that catalyzes the reduction of dihydro-4-hydroxyphenylpyruvate (HHPP) to generate tetrahydro-4-hydroxyphenylpyruvate (HHPP), which is the third step of the biosynthetic pathway from prephenate to Choi. Comparative structural analysis indicates that ligand binding-induced conformational change of AerF is different from that of the other SDR superfamily reductase using HHPP as a substrate. Analyses of NADPH and substrate analogue binding sites combined with the results of mutagenesis analyses suggest that a particular serine residue mainly involves in the initiation of the proton transfer between the substrate and the residues of AerF, which is an uncommon feature in SDR superfamily reductase. Furthermore, based on the observations of structural and mutagenesis analyses, the catalytic mechanism of AerF is proposed and a proton transfer pathway in AerF is deduced.

PubMed: 31726097
DOI: 10.1016/j.jsb.2019.107415

実験手法

X-RAY DIFFRACTION (1.78 Å)