6JG9

Crystal structure of AimR in complex with arbitrium peptide

6JG9 の概要

• エントリーDOI: 10.2210/pdb6jg9/pdb
• 分子名称: AimR transcriptional regulator, arbitrium peptide (3 entities in total)
• 機能のキーワード: aimr, apo, hth, peptide binding protein, peptide binding protein-peptide complex, peptide binding protein/peptide
• 由来する生物種: Bacillus phage SPbeta (Bacillus phage SPBc2); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 94327.98

構造登録者

Guan, Z.Y.,Pei, K.,Zou, T.T. (登録日: 2019-02-13, 公開日: 2019-07-03, 最終更新日: 2023-11-22)

主引用文献

Guan, Z.Y.,Pei, K.,Wang, J.,Cui, Y.Q.,Zhu, X.,Su, X.,Zhou, Y.B.,Zhang, D.L.,Tang, C.,Yin, P.,Liu, Z.,Zou, T.T.
Structural insights into DNA recognition by AimR of the arbitrium communication system in the SPbeta phage.
Cell Discov, 5:29-29, 2019

PubMed Abstract: A newly identified arbitrium communication system regulates the lysis-to-lysogeny decision in a bacteriophage. This system contains an arbitrium hexapeptide as a signal, the cellular receptor AimR, and the lysogenic negative regulator AimX. AimR specifically targets the downstream DNA to activate gene expression. The arbitrium peptide binds to AimR, inhibiting its DNA-binding to promote phage lysogeny. Recently, we and other groups have elucidated how arbitrium peptide sensed by AimR. However, the molecular mechanisms of DNA recognition by AimR and the regulation of its DNA-binding activity by the peptide remain largely unknown. Here, we report the crystal structure of the AimR-DNA complex at 2.1 Å resolution. The N-terminal HTH motif recognizes the palindromic DNA sequence, buttressed by interactions between positively charged residues and the DNA phosphate groups. The DNA-bound AimR assembles a more closed dimer than the peptide-bound form. Single-molecule FRET and crosslinking assays revealed that the AimR protein samples both open and closed conformations in solution. Arbitrium peptide binding induces a closed-to-open conformational change of AimR, eliminating DNA targeting. Our structural and functional analysis provides new insights into the DNA recognition mechanism of AimR and its regulation by the arbitrium peptide in the context of phage lysis-lysogeny decisions.

PubMed: 31149347
DOI: 10.1038/s41421-019-0101-2

実験手法

X-RAY DIFFRACTION (1.998 Å)