6JEE

Crystal structure of apo-L161C/L165C-Fr

6JEE の概要

• エントリーDOI: 10.2210/pdb6jee/pdb
• 分子名称: Ferritin light chain, CADMIUM ION, SULFATE ION, ... (6 entities in total)
• 機能のキーワード: metal binding protein
• 由来する生物種: Equus caballus (Horse)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 20766.17

構造登録者

Abe, S.,Ito, N.,Maity, B.,Lu, C.,Lu, D.,Ueno, T. (登録日: 2019-02-05, 公開日: 2019-12-11, 最終更新日: 2023-11-22)

主引用文献

Abe, S.,Ito, N.,Maity, B.,Lu, C.,Lu, D.,Ueno, T.
Coordination design of cadmium ions at the 4-fold axis channel of the apo-ferritin cage.
Dalton Trans, 48:9759-9764, 2019

PubMed Abstract: Spherical protein cages with highly symmetrical structures provide unique environments for the conjugation of metal ions and metal nanoparticles. Ferritin has been widely studied as a template for the coordination of metal ions and metal nanoparticles in fundamental research and applications. However, it remains difficult to design metal coordination sites precisely. In this work, we describe the design and construction of new metal coordination sites by introducing Cys residues at the 4-fold symmetrical hydrophobic channel of apo-ferritin. X-ray crystal structure analyses of the mutants containing Cd(ii) ions show that the four or eight binding sites for Cd(ii) ions are located at the 4-fold symmetrical axis channel of apo-ferritin. It was found that the coordination number and configuration of Cd(ii) ions can be varied by adjusting the positions of the Cys residues at the symmetrical channels of the apo-ferritin cage.

PubMed: 30993287
DOI: 10.1039/c9dt00609e

実験手法

X-RAY DIFFRACTION (1.3 Å)