6JBM

Crystal structure of the TRIM14 PRYSPRY domain

6JBM の概要

• エントリーDOI: 10.2210/pdb6jbm/pdb
• 分子名称: Tripartite motif-containing protein 14, SULFATE ION (3 entities in total)
• 機能のキーワード: trim, protein interaction domain, protein binding
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 92729.31

構造登録者

Yin, Y.X.,Yu, Y.,Liang, L. (登録日: 2019-01-26, 公開日: 2020-03-04, 最終更新日: 2023-11-22)

主引用文献

Yu, Y.,Liang, L.,Jin, Y.,Yin, Y.
The TRIM14 PRYSPRY domain mediates protein interaction via its basic interface.
Febs Lett., 593:1122-1129, 2019

PubMed Abstract: Tripartite motif (TRIM)14 was recently shown to be an important molecule against pathogens. Its PRYSPRY domain acts as a protein-protein interaction module. TRIM14 exerts distinct functions via its PRYSPRY domain by interacting with different partners. However, the structural basis for its binding specificity remains unknown. Here we solved the crystal structure of the TRIM14 PRYSPRY domain, and found a positively charged surface that may mediate its partner specificity. Isothermal titration calorimetry reveals that the TRIM14 PRYSPRY domain binds to acidic peptides, and the analysis of the reported partners of TRIM14 is consistent with our assumption. Therefore, we demonstrate that the PRYSPRY domain of TRIM14 harbors a putative basic interface that may favorably bind to acidic amino acid residues.

PubMed: 30973643
DOI: 10.1002/1873-3468.13386

実験手法

X-RAY DIFFRACTION (2.1 Å)