6J8M

Low-dose structure of bovine heart cytochrome c oxidase in the fully oxidized state determined using 30 keV X-ray

6J8M の概要

• エントリーDOI: 10.2210/pdb6j8m/pdb
• 分子名称: Cytochrome c oxidase subunit 1, Cytochrome c oxidase subunit 7A1, Cytochrome c oxidase subunit 7B, ... (28 entities in total)
• 機能のキーワード: oxidoreductase, proton pump, heme, respiratory chain
• 由来する生物種: Bos taurus (Bovine); 詳細
• タンパク質・核酸の鎖数: 26
• 化学式量合計: 442865.98

構造登録者

Ueno, G.,Shimada, A.,Yamashita, E.,Hasegawa, K.,Kumasaka, T.,Shinzawa-Itoh, K.,Yoshikawa, S.,Tsukihara, T.,Yamamoto, M. (登録日: 2019-01-20, 公開日: 2019-06-26, 最終更新日: 2023-11-22)

主引用文献

Ueno, G.,Shimada, A.,Yamashita, E.,Hasegawa, K.,Kumasaka, T.,Shinzawa-Itoh, K.,Yoshikawa, S.,Tsukihara, T.,Yamamoto, M.
Low-dose X-ray structure analysis of cytochrome c oxidase utilizing high-energy X-rays.
J.Synchrotron Radiat., 26:912-921, 2019

PubMed Abstract: To investigate the effect of high-energy X-rays on site-specific radiation-damage, low-dose diffraction data were collected from radiation-sensitive crystals of the metal enzyme cytochrome c oxidase. Data were collected at the Structural Biology I beamline (BL41XU) at SPring-8, using 30 keV X-rays and a highly sensitive pixel array detector equipped with a cadmium telluride sensor. The experimental setup of continuous sample translation using multiple crystals allowed the average diffraction weighted dose per data set to be reduced to 58 kGy, and the resulting data revealed a ligand structure featuring an identical bond length to that in the damage-free structure determined using an X-ray free-electron laser. However, precise analysis of the residual density around the ligand structure refined with the synchrotron data showed the possibility of a small level of specific damage, which might have resulted from the accumulated dose of 58 kGy per data set. Further investigation of the photon-energy dependence of specific damage, as assessed by variations in UV-vis absorption spectra, was conducted using an on-line spectrometer at various energies ranging from 10 to 30 keV. No evidence was found for specific radiation damage being energy dependent.

PubMed: 31274413
DOI: 10.1107/S1600577519006805

実験手法

X-RAY DIFFRACTION (1.9 Å)