6J7K

Crystal structure of Pseudomonas aeruginosa Earp in complex with TDP-Rha

6J7K の概要

• エントリーDOI: 10.2210/pdb6j7k/pdb
• 分子名称: Pseudomonas aeruginosa Earp, 2'-DEOXY-THYMIDINE-BETA-L-RHAMNOSE, 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, ... (4 entities in total)
• 機能のキーワード: rhamnosyltransferase, transferase
• 由来する生物種: Pseudomonas aeruginosa PAO1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 47062.31

構造登録者

He, C.,Li, F. (登録日: 2019-01-18, 公開日: 2019-05-15, 最終更新日: 2024-10-09)

主引用文献

He, C.,Liu, N.,Li, F.,Jia, X.,Peng, H.,Liu, Y.,Xiao, Y.
Complex Structure ofPseudomonas aeruginosaArginine Rhamnosyltransferase EarP with Its Acceptor Elongation Factor P.
J.Bacteriol., 201:-, 2019

PubMed Abstract: A bacterial inverting glycosyltransferase EarP transfers rhamnose from dTDP-β-l-rhamnose (TDP-Rha) to Arg32 of translation elongation factor P (EF-P) to activate its function. We report here the structural and biochemical characterization of EarP. In contrast to recently reported EarP, EarP exhibits differential conformational changes upon TDP-Rha and EF-P binding. Sugar donor binding enhances acceptor binding to EarP, as revealed by structural comparison between the apo-, TDP-Rha-, and TDP/EF-P-bound forms and isothermal titration calorimetry experiments. EF-P rhamnosylation combined with active-site geometry indicates that Asp16 corresponding to Asp20 of EarP is the catalytic base, whereas Glu272 is another putative catalytic residue. Our study should provide the basis for EarP-targeted inhibitor design against infections from and other clinically relevant species. Posttranslational rhamnosylation of EF-P plays a key role in , establishing virulence and antibiotic resistance, as well as survival. The detailed structural and biochemical characterization of the EF-P-specific rhamnosyltransferase EarP from not only demonstrates that sugar donor TDP-Rha binding enhances acceptor EF-P binding to EarP but also should provide valuable information for the structure-guided development of its inhibitors against infections from and other EarP-containing pathogens.

PubMed: 31010899
DOI: 10.1128/JB.00128-19

実験手法

X-RAY DIFFRACTION (2.15 Å)