6J4Z

RNA polymerase II elongation complex bound with Spt4/5 and foreign DNA, stalled at SHL(-1) of the nucleosome

6J4Z の概要

• エントリーDOI: 10.2210/pdb6j4z/pdb
• EMDBエントリー: 0674
• 分子名称: DNA-directed RNA polymerase subunit, RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III, RNA polymerase II subunit B12.5, ... (25 entities in total)
• 機能のキーワード: nucleosome, chromatin, rna polymerase, transcription, transcription-rna-dna complex, transcription/rna/dna
• 由来する生物種: Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast); 詳細
• タンパク質・核酸の鎖数: 27
• 化学式量合計: 889692.38

構造登録者

Ehara, H.,Kujirai, T.,Fujino, Y.,Shirouzu, M.,Kurumizaka, H.,Sekine, S. (登録日: 2019-01-10, 公開日: 2019-02-20, 最終更新日: 2024-03-27)

主引用文献

Ehara, H.,Kujirai, T.,Fujino, Y.,Shirouzu, M.,Kurumizaka, H.,Sekine, S.I.
Structural insight into nucleosome transcription by RNA polymerase II with elongation factors.
Science, 363:744-747, 2019

PubMed Abstract: RNA polymerase II (RNAPII) transcribes chromosomal DNA that contains multiple nucleosomes. The nucleosome forms transcriptional barriers, and nucleosomal transcription requires several additional factors in vivo. We demonstrate that the transcription elongation factors Elf1 and Spt4/5 cooperatively lower the barriers and increase the RNAPII processivity in the nucleosome. The cryo-electron microscopy structures of the nucleosome-transcribing RNAPII elongation complexes (ECs) reveal that Elf1 and Spt4/5 reshape the EC downstream edge and intervene between RNAPII and the nucleosome. They facilitate RNAPII progression through superhelical location SHL(-1) by adjusting the nucleosome in favor of the forward progression. They suppress pausing at SHL(-5) by preventing the stable RNAPII-nucleosome interaction. Thus, the EC overcomes the nucleosomal barriers while providing a platform for various chromatin functions.

PubMed: 30733384
DOI: 10.1126/science.aav8912

実験手法

ELECTRON MICROSCOPY (4.1 Å)