6J2N

yeast proteasome in substrate-processing state (C3-b)

これはPDB形式変換不可エントリーです。

6J2N の概要

• エントリーDOI: 10.2210/pdb6j2n/pdb
• EMDBエントリー: 9769 9770
• 分子名称: Proteasome subunit beta type-1, Proteasome subunit alpha type-3, Proteasome subunit alpha type-4, ... (33 entities in total)
• 機能のキーワード: proteasome, k48-ub4, ub-bound, cryo-em, hydrolase
• 由来する生物種: Saccharomyces cerevisiae S288c (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 47
• 化学式量合計: 1692659.85

構造登録者

Cong, Y. (登録日: 2019-01-02, 公開日: 2019-03-20, 最終更新日: 2024-10-23)

主引用文献

Ding, Z.,Xu, C.,Sahu, I.,Wang, Y.,Fu, Z.,Huang, M.,Wong, C.C.L.,Glickman, M.H.,Cong, Y.
Structural Snapshots of 26S Proteasome Reveal Tetraubiquitin-Induced Conformations.
Mol. Cell, 73:1150-, 2019

PubMed Abstract: The 26S proteasome is the ATP-dependent protease responsible for regulating the proteome of eukaryotic cells through degradation of mainly ubiquitin-tagged substrates. In order to understand how proteasome responds to ubiquitin signal, we resolved an ensemble of cryo-EM structures of proteasome in the presence of K48-Ub, with three of them resolved at near-atomic resolution. We identified a conformation with stabilized ubiquitin receptors and a previously unreported orientation of the lid, assigned as a Ub-accepted state C1-b. We determined another structure C3-b with localized K48-Ub to the toroid region of Rpn1, assigned as a substrate-processing state. Our structures indicate that tetraUb induced conformational changes in proteasome could initiate substrate degradation. We also propose a CP gate-opening mechanism involving the propagation of the motion of the lid to the gate through the Rpn6-α2 interaction. Our results enabled us to put forward a model of a functional cycle for proteasomes induced by tetraUb and nucleotide.

PubMed: 30792173
DOI: 10.1016/j.molcel.2019.01.018

実験手法

ELECTRON MICROSCOPY (7.5 Å)