6J0M

Cryo-EM Structure of an Extracellular Contractile Injection System, PVC baseplate in extended state (reconstructed with C3 symmetry)

6J0M の概要

• エントリーDOI: 10.2210/pdb6j0m/pdb
• EMDBエントリー: 9764
• 分子名称: Pvc8 (1 entity in total)
• 機能のキーワード: assembly, photorhabdus asymbiotica, pvc, contractile injection system, bacteriophage-like, protein transport
• 由来する生物種: Photorhabdus asymbiotica
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 180089.11

構造登録者

Jiang, F.,Li, N.,Wang, X.,Cheng, J.,Huang, Y.,Yang, Y.,Yang, J.,Cai, B.,Wang, Y.,Jin, Q.,Gao, N. (登録日: 2018-12-24, 公開日: 2019-04-10, 最終更新日: 2024-03-27)

主引用文献

Jiang, F.,Li, N.,Wang, X.,Cheng, J.,Huang, Y.,Yang, Y.,Yang, J.,Cai, B.,Wang, Y.P.,Jin, Q.,Gao, N.
Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System.
Cell, 177:370-383.e15, 2019

PubMed Abstract: Contractile injection systems (CISs) are cell-puncturing nanodevices that share ancestry with contractile tail bacteriophages. Photorhabdus virulence cassette (PVC) represents one group of extracellular CISs that are present in both bacteria and archaea. Here, we report the cryo-EM structure of an intact PVC from P. asymbiotica. This over 10-MDa device resembles a simplified T4 phage tail, containing a hexagonal baseplate complex with six fibers and a capped 117-nanometer sheath-tube trunk. One distinct feature of the PVC is the presence of three variants for both tube and sheath proteins, indicating a functional specialization of them during evolution. The terminal hexameric cap docks onto the topmost layer of the inner tube and locks the outer sheath in pre-contraction state with six stretching arms. Our results on the PVC provide a framework for understanding the general mechanism of widespread CISs and pave the way for using them as delivery tools in biological or therapeutic applications.

PubMed: 30905475
DOI: 10.1016/j.cell.2019.02.020

実験手法

ELECTRON MICROSCOPY (3.9 Å)