6IWV

Crystal structure of a single strand DNA binding protein

6IWV の概要

• エントリーDOI: 10.2210/pdb6iwv/pdb
• 分子名称: Single-stranded DNA-binding protein 2 (2 entities in total)
• 機能のキーワード: a single strand dna binding protein, dna binding protein
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 15709.72

構造登録者

Wang, H.Y.,Yan, X.X. (登録日: 2018-12-07, 公開日: 2019-08-14, 最終更新日: 2024-03-27)

主引用文献

Wang, H.,Wang, Z.,Tang, Q.,Yan, X.X.,Xu, W.
Crystal structure of the LUFS domain of human single-stranded DNA binding Protein 2 (SSBP2).
Protein Sci., 28:788-793, 2019

PubMed Abstract: The human single-stranded DNA binding Protein 2 (SSBP2) is a tumor suppressor implicated in multiple cancer forms. The SSBP2 and related SSBP3/SSBP4 proteins are predicted to be intrinsically disordered excepted for their highly conserved N-terminal LUFS (LUG/LUH, Flo8, and SSBP/SSDP) domain. LUFS domains are found in a number of proteins including some transcriptional co-repressors. Although LUFS domains contain an N-terminal Lis homology (LisH) motif that typically forms a stable dimer, no 3D structure of any LUFS domain is available. Here, we report a crystal structure of the LUFS domain of human SSBP2 at 1.52 Å resolution. We show that the SSBP2 LUFS domain forms a homo-tetramer and reveal how an alpha-helix C-terminal to the LisH motif mediates SSBP2 tetramerization (dimerization of dimers). Conservation of the tetramerization interface among LUFS domains suggests that other LUFS domains may also form tetramers in similar manners.

PubMed: 30676665
DOI: 10.1002/pro.3581

実験手法

X-RAY DIFFRACTION (1.52 Å)